Mechanical stimulation was performed as previously described41 . Briefly, a 685g rubber weight was dropped onto a rack supporting small DAMs monitors (Trikinetics, wide awake) or MultiBeam Activity Monitors (Trikinetics, AβArctic overexpression) at 12AM, 3AM, and 6AM. The absence of activity 5 min before a stimulus was counted as a sleep episode, and flies exhibiting beam crossings within 2 min after the stimulation were recorded as “aroused”. We detected no differences in arousal within an experimental condition across the time points.
Multibeam activity monitors
Manufactured by Trikinetics
The MultiBeam Activity Monitors are a line of laboratory equipment designed to track and record the movement and activity of small animals. The core function of these monitors is to detect and quantify the locomotor activity of the subjects under observation using multiple infrared beams.
Automatically generated - may contain errors
3 protocols using multibeam activity monitors
1
Mechanical Stimulation Induces Fly Arousal
2
Drosophila Sleep Monitoring Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult female flies were collected 2 to 3 days after eclosion and aged in group housing on standard food at 25°C on a 12-hour:12-hour LD cycle (unless otherwise noted). Flies aged 5 to 7 days were anesthetized on CO2 pads (Genesee Scientific, catalog no. 59-114) and loaded into individual glass tubes (with 5% sucrose and 2% agar) for monitoring locomotor activity in the Drosophila Activity Monitoring (DAM) system (TriKinetics, Waltham, MA) or MultiBeam Activity Monitors (Trikinetics, Waltham, MA) as denoted in figure legends. All sleep experiments were loaded between ZT5-ZT10. Data collection began at ZT0 at least 24 hours following CO2 anesthesia. Activity was measured in 1-min bins, and sleep was defined as 5 min of consolidated inactivity (31 (link), 32 (link)). Data processing was performed using PySolo (73 (link)). Mechanical sleep deprivation was performed using a Trikinetics vortexer mounting plate. Monitors were shaken for 2 s randomly within every 20-s window for 12 hours during the night. Rebound sleep was calculated as the difference between baseline sleep duration for 12 hours during the day preceding deprivation and rebound sleep duration for 12 hour during the day immediately following nighttime deprivation.
3
Mechanical Stimulation Induces Fly Arousal
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mechanical stimulation was performed as previously described41 . Briefly, a 685g rubber weight was dropped onto a rack supporting small DAMs monitors (Trikinetics, wide awake) or MultiBeam Activity Monitors (Trikinetics, AβArctic overexpression) at 12AM, 3AM, and 6AM. The absence of activity 5 min before a stimulus was counted as a sleep episode, and flies exhibiting beam crossings within 2 min after the stimulation were recorded as “aroused”. We detected no differences in arousal within an experimental condition across the time points.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!