Prednisone (CAS #53-03-2, PN. MB1327) was obtained from MeiLunbio Co., LTD. (Dalian, Liaoning, China). Isoflurane was obtained from Baxter Healthcare Co. (Deerfield, IL, USA). The antibodies of proliferating cell nuclear antigen (PCNA, No. A0264) and mouse vasa homolog (MVH, No. A15624) were purchased from ABclonal Technology Co., Ltd. (Wuhan, Hubei, China). All the primers were synthesized by TIANYIHUIYUAN Biotechnology Co., Ltd. (Wuhan, Hubei, China). The enzyme-linked immunosorbent assay (ELISA) kit was obtained from the Beijing North Institute of Biological Technology (Beijing, China). The total RNA Trizol kit was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Reverse transcription and quantitative real-time polymerase chain reaction (RT-qPCR) kits (Q223) were purchased from Takara Biotechnology Co., Ltd. (Dalian, Liaoning, China). Other chemicals and reagents were of analytical grade.
Rt qpcr kits q223
Manufactured by Takara Bio
Sourced in China
The RT-qPCR kits (Q223) from Takara Bio are designed for reverse transcription and real-time quantitative PCR (RT-qPCR) analysis. The kits provide the necessary reagents and enzymes to perform reverse transcription and quantitative PCR in a single reaction.
Automatically generated - may contain errors
2 protocols using rt qpcr kits q223
1
Prednisone and Reproductive Cellular Markers
2
Methomyl and Folic Acid Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methomyl and folic acid were obtained from Sigma (St. Louis, MO). The CellTiter96® AQueous cell proliferation assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MTS) was from Promega (Madison, WI, USA). Mouse iodine (125I) testosterone radioimmunoassay kits (S1094093) were purchased from the North Institute of Biological Technology (Beijing, China). The total RNA TRIzol kit was purchased from Omega Bio-Tek (Doraville, Georgia, USA). Reverse transcription and quantitative real-time PCR (RT-qPCR) kits (Q223) were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). The primers were synthesized by TIANYIHUIYUAN Biotechnology Co., Ltd. (Wuhan, China). The other reagents for experiments were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!