Human LAD cell lines (A549, SPC-A1, H1299 and PC-9) and normal lung epithelial cells BEAS-2B were bought from the Cell Bank of the Chinese Academy of Sciences and incubated with Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen Life Technology Inc., Carlsbad, CA), containing 10% fetal bovine serum (FBS) with 5% CO2 at 37 °C in humid air. All cell lines were authenticated via STR profiling before using.
The suppression of LINC02418 expression was achieved by sh-LINC02418#1/2. Sh-LINC02418#1 and sh-LINC02418#2 were obtained from GenePharma (Shanghai, China). LINC02418 and KNL1 were overexpressed with pcDNA3.1 vectors (Invitrogen, Carlsbad, USA). MiR-4677-3p mimics were applied to elevate miR-4677-3p expression. MiR-4677-3p mimics and NC mimics were also bought from GenePharma (Shanghai, China). The transfection of above plasmids was conducted by use of Lipofectamine® 2000 agent (Invitrogen).
The suppression of LINC02418 expression was achieved by sh-LINC02418#1/2. Sh-LINC02418#1 and sh-LINC02418#2 were obtained from GenePharma (Shanghai, China). LINC02418 and KNL1 were overexpressed with pcDNA3.1 vectors (Invitrogen, Carlsbad, USA). MiR-4677-3p mimics were applied to elevate miR-4677-3p expression. MiR-4677-3p mimics and NC mimics were also bought from GenePharma (Shanghai, China). The transfection of above plasmids was conducted by use of Lipofectamine® 2000 agent (Invitrogen).