The 293T cells transfected with Gas-miR01/Gas-miR02/NC mimics were collected, and then the total proteins were extracted using the Cell Lysis Reagent (Roche). Total proteins were separated by 12% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, USA) by electroblotting. After blocking with 5% skim milk in tris-buffered saline with Tween (TBST) (pH 7.4) for 2 h at room temperature, the protein was detected with an anti-A20 antibody (Cell Signaling Technology, USA) overnight at 4°C. The membrane was washed with TBST, and HRP-labeled (horseradish peroxidase-labeled) mouse anti-rabbit antibody was used as the secondary antibody (Cell Signaling Technology, USA). The signals were detected using the ECL Detection Kit (Advansta).
Anti a20 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-A20 antibody is a laboratory tool used to detect and study the A20 protein, which is a key regulator of inflammatory and immune responses. This antibody specifically binds to the A20 protein, allowing researchers to investigate its expression, localization, and interactions within cells and tissues.
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Lab products found in correlation
3 protocols using anti a20 antibody
1
Western Blot Analysis of A20 Protein
2
Co-Immunoprecipitation of ABIN1 and A20
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The peri-infarct cortex was homogenized in IP lysis buffer (Beyotime, China) to extract the proteins. One microgram of anti-ABIN1 antibody (#4664, Cell Signaling Technology), anti-A20 antibody (#5630, Cell Signaling Technology), or normal rat IgG was added to each milligram of total protein in the supernatants, and the samples were rotated at 4°C overnight. Forty microliters of protein A/G agarose beads was mixed with the supernatants and rotated at 4°C for 2 h. The beads were washed with lysis buffer and then eluted with 40 μl of SDS loading buffer. The supernatants were collected for western blot.
3
Ubiquitination Pathway Regulates CXCL10 Expression
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