Fluorescence emission spectra from aqueous nanotube solutions were acquired with a home- built spectroscopy system as described19 . Briefly, carbon nanotube samples were assayed in a 96 well plate format on an inverted microscope using a tunable white-light laser (NKT Photonics) coupled to a variable bandpass filter. Emission light was directed into a spectrometer with a focal length of 320 mm and aperture ratio of f/4.6 (Princeton Instruments IsoPlane SCT 320) and InGaAs array camera (Princeton Instruments 640 × 512 pixel NIRvana). For all experiments, measurements were taken in triplicate across three wells. Following spectra acquisition, custom code written in Matlab applied spectral corrections and background subtraction as described19 . Corrected spectra were used to fit the data with Voigt functions. Error bars and linear fits were computed with GraphPad Prism 6.
Isoplane sct 320
Manufactured by Teledyne
Sourced in United States
The IsoPlane SCT 320 is a high-performance spectrograph designed for use in a variety of applications. It features a Czerny-Turner optical layout and a two-dimensional CCD detector, providing efficient light collection and high-resolution spectral data. The instrument is capable of covering a wide spectral range and can be configured with different gratings to meet specific experimental requirements.
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Lab products found in correlation
Dv897dcs bv, by Oxford Instruments (1 mentions)
Pixis 400br, by Teledyne (1 mentions)
Pm100d, by Thorlabs (1 mentions)
Gl10 b, by Thorlabs (1 mentions)
Cvh100, by Thorlabs (1 mentions)
Neo 5, by Oxford Instruments (1 mentions)
Rms4x, by Olympus (1 mentions)
Proem, by Teledyne (1 mentions)
Nanolab 600 dual beam fib sem, by Thermo Fisher Scientific (1 mentions)
Halogen lamp, by Olympus (1 mentions)
6 protocols using isoplane sct 320
1
Fluorescence Spectroscopy of Aqueous Nanotubes
2
Imaging Upconversion Nanoparticles in Lymph Nodes
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The UCNP solutions were excited by a 980 nm continuous-wave (CW) single-mode diode laser (P161-600-980A, EM4, Andor Technology, Belfast, UK). Their emission was collected through an optical fibre by a home-made UCL spectrograph and imaging system, which was composed of an inverted microscope (IX71, Olympus, Tokyo, Japan) and an electron multiplying charge coupled device (EMCCD) camera (DV897DCS-BV, iXon, Andor Technology, Belfast, UK). It was detected by a CCD camera (PIXIS 400BR, Princeton Instruments, Trenton, NJ, USA) attached to an imaging spectrograph (IsoPlane SCT320, Princeton Instruments). The images of individual UCNPs or UCNPs in sliced SLN tissues were taken from the same imaging system. To obtain colour images of SLN tissues, a colour sCMOS camera (OS4MPc-CL-RGB, Raptor Photonics, Larne, UK) was employed.
3
Upconversion Nanoparticle Emission Spectroscopy
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Downconverted emission spectra were recorded using a spectrograph (Princeton Instruments, NJ, USA, Isoplane SCT320) equipped with a cooled IR camera (Princeton Instruments, NJ, USA). The UCNP samples were placed into the cuvette holder (Thorlabs Inc., Newton, MA, USA, CVH100) equipped with a 1050 nm long-pass edge filter (Thorlabs Inc., Newton, MA, USA, FELH1050) and an optical collimator and then connected to the spectrograph. Samples were excited using a Ti:sapphire 980 nm laser (Spectra-Physics, Santa Clara, CA, USA, Mai Tai) with a pulse width = 100 fs and repetition rate = 80 MHz. The laser output beam was expanded with a 5× beam expander (Thorlabs Inc., Newton, MA, USA, GBE05-B) and attenuated by a variable iris diaphragm at approximately 0.5 cm in diameter. The intensity of the laser beam was controlled by rotating a half-wave plate (Thorlabs Inc., Newton, MA, USA; AHWP10M-980) mounted at the front of a laser-Glan polarizer (Thorlabs Inc., Newton, MA, USA, GL10-B) and attenuated to a 135 mW average power. The intensity of the beam was monitored by a digital power meter (Thorlabs Inc., Newton, MA, USA, PM100D) equipped with thermal power sensors (Thorlabs Inc., Newton, MA, USA, S470C).
4
Nonlinear Optical Characterization of Metasurface
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The femtosecond laser (Coherent, Astrella, repetition rate: 1 kHz) pumped OPA (Coherent OPerA Solo) output at a wavelength of 1220 nm was used as the pump laser. The laser was split into two beams with H- and V-polarizations by a polarization beam splitter (PBS). The power of the two beams was equalized by adjusting a half-wave plate (λ/2). A variable time delay was introduced into the H-polarized beam by using a delay line (Newport DLS225). The two beams were collinearly combined with the second PBS and focused onto the metasurface sample with a lens (focal length: 400 mm). The transmitted pump waves and SH wave were collected by a 4× infinity-corrected plan achromatic objective lens (Olympus RMS4X). After that, the pump beams were blocked by the short pass filters. The polarization state of the SH wave is analyzed by using a linear polarizer. The flip mirror was moved out of the optical path for characterizing the spectra of the SH wave and moved in for capturing the SHG image of dual-channel metasurface with a scientific complementary metal-oxide semiconductor camera (Andor Neo 5.5). The spectra and power of the SH waves were measured by using the spectrometer (Princeton Instruments IsoPlane SCT320) equipped with an EMCCD detector (Princeton Instruments ProEM).
5
Plasmonic Device Fabrication and Characterization
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For our study plasmonic devices based on the design in Fig. 1 were fabricated in a 150 nm thick Ag film with a 3 nm Ge adhesion layer. Both layers were deposited by electron-beam evaporation (Nanochrome II, Intlvac) at a 3 Å/s deposition rate on a semiconductor grade quartz substrate. Focused Ion Beam (FIB) lithography (Helios NanoLab 600 Dual Beam FIB-SEM, FEI) was employed to mill the apertures using 9.7 pA currents. Optical images were collected using an inverted optical microscope (Nikon Ti-U) at x100 magnification utilising a broad-band Xenon light source. Transmission spectra were acquired using the same set-up, employing a spectral analyser (IsoPlane SCT 320, Princeton Instruments) at 1200 gratings/mm. All spectra were normalized with respect to the bare quartz substrate using the patterned area as the region of interest.
6
Transmission Spectroscopy of Microscopic Samples
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Transmission spectra were recorded using an Olympus inverted microscope (IX83 series), in bright-field (BF) mode, with light source of halogen lamp (100 W, Olympus). The spectra were collected with a 40Â magnification (NA = 0.6) objective, and were normalized to the transmission spectrum of a glass substrate under the same optical conditions (in half of the exposure time). The microscope was coupled to a spectrophotometer (IsoPlane SCT-320, Princeton Instruments) and to a charge-coupled device camera (CCD, PIXS1024b, Princeton Instruments).
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