Milliplex map mouse adipokine magnetic bead panel

Manufactured by Merck Group

Sourced in United States

The MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel is a multiplex assay kit designed for the simultaneous quantitative measurement of multiple adipokines in mouse samples using the Luminex technology. The panel includes magnetic beads coated with specific capture antibodies for each target analyte, allowing for the detection and quantification of various adipokines in a single experiment.

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Lab products found in correlation

Mouse adiponectin elisa kit, by Merck Group (1 mentions) Dimension xpand plus, by Siemens (1 mentions) Gip elisa, by Merck Group (1 mentions) Luminex 200 system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel - Endocrine Multiplex Assay (4 citations) MADKMAG-71K Milliplex map mouse adipokine magnetic bead panel—endocrine multiplex assay (2 citations) MILLIPLEX® Mouse Adipokine Magnetic Bead Panel (3 citations) Mouse Adipokine Magnetic Bead Panel (2 citations) MILLIPLEX MAP (123 citations) Milliplex panels (4 citations) Magnetic beads (109 citations)

5 protocols using milliplex map mouse adipokine magnetic bead panel

Measurement of Metabolic Biomarkers in Mice

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Collected serum samples were sent to Hematology & Biochemistry Clinical Laboratory, Veterinary Laboratory Service Unit, Faculty of Veterinary Medicine, University Putra Malaysia, for measurement of fasting blood glucose, total cholesterol, triglycerides, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) using Dimension Xpand Plus clinical chemistry system (SIEMENS Healthcare Diagnostics Inc.).
Levels of different adipokines (TNF-α, monocyte chemotactic protein 1 (MCP-1), leptin, insulin, interleukin-6 (IL-6) and resistin) in mouse sera were measured using the MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel (Millipore, Billerica, MA). Serum adiponectin level was measured by commercially available mouse adiponectin ELISA kit, (Millipore, Billerica, MA) according to manufacturer’s protocol.

Khalilpourfarshbafi M., Devi Murugan D., Abdul Sattar M.Z., Sucedaram Y, & Abdullah N.A. (2019). Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice. PLoS ONE, 14(6), e0218792.

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Serum Analysis of Insulin, Adipokines

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To analyze serum, we conducted insulin ELISA (Morinaga), GLP-1 (Active) ELISA (Shibayagi), GIP ELISA (Millipore), and adiponectin ELISA (Otsuka). To detect adipokines, namely, leptin, tissue plasminogen activator inhibitor-1 (tPAI-1), resistin, and MCP-1, a MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel (Millipore) was used.

Kihira Y., Miyake M., Hirata M., Hoshina Y., Kato K., Shirakawa H., Sakaue H., Yamano N., Izawa-Ishizawa Y., Ishizawa K., Ikeda Y., Tsuchiya K., Tamaki T, & Tomita S. (2014). Deletion of Hypoxia-Inducible Factor-1α in Adipocytes Enhances Glucagon-Like Peptide-1 Secretion and Reduces Adipose Tissue Inflammation. PLoS ONE, 9(4), e93856.

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Plasma Cytokine Profiling in PCB-Exposed Mice

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Plasma cytokine levels were measured using the Milliplex Map Mouse Adipokine Magnetic Bead Panel (Millipore Corp, Billerica, MA, USA) on the Luminex Xmap MAGPIX system (Luminex Corp, Austin, TX, USA), as per the manufacturer’s instructions. Only PAI-1 levels were statistically altered in PCB-exposed mice.

Petriello M.C., Hoffman J.B., Sunkara M., Wahlang B., Perkins J.T., Morris A.J, & Hennig B. (2016). Dioxin-like pollutants increase hepatic flavin containing monooxygenase (FMO3) expression to promote synthesis of the pro-atherogenic nutrient biomarker Trimethylamine N-oxide from dietary precursors. The Journal of nutritional biochemistry, 33, 145-153.

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Luminex-Based Cytokine Profiling

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Serum cytokine/chemokine protein levels were assayed with a MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel (Millipore, Burlington, MA) according to the manufacturer’s recommendations on a Luminex 200 System (Life Technologies) and analyzed using the xPONENT software.

Khatua B., El-Kurdi B., Patel K., Rood C., Noel P., Crowell M., Yaron J.R., Kostenko S., Guerra A., Faigel D.O., Lowe M, & Singh V.P. (2021). Adipose saturation reduces lipotoxic systemic inflammation and explains the obesity paradox. Science Advances, 7(5), eabd6449.

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Multiplex Assay for Plasma Cytokines and CVD Markers

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The Milliplex Map Mouse Adipokine Magnetic Bead Panel (Millipore Corp, Billerica, MA, USA) was utilized to measure plasma cytokines (tumor necrosis factor alpha (Tnfα), interleukin-6 (IL-6), macrophage chemoattractant protein-1 (Mcp-1)), insulin, adipokines (leptin, resistin), and plasminogen activator inhibitor-1 (PAI-1); while the Milliplex Map Mouse CVD Magnetic Bead Panel 1 was used to measure plasma CVD markers on the Luminex Xmap MAGPIX system (Luminex Corp, Austin, TX, USA), as per the manufacturer’s instructions.

Wahlang B., Perkins J.T., Petriello M.C., Hoffman J.B., Stromberg A.J, & Hennig B. (2017). A Compromised Liver Alters Polychlorinated Biphenyl-Mediated Toxicity. Toxicology, 380, 11-22.

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