MV4-11, MOLM-13, OCI-AML2, and OCI-AML3 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MV4-11 and MOLM-13 expressed MLL fusion oncoprotein with FLT3-ITD. OCI-AML2 and OCI-AML3 expressed mutant NPM1c+ THP1 without FLT3-ITD or NPM1c + mutant, all cells were cultured in RPMI 1640 with 20% fetal bovine serum (Gibco).
Oci aml2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The OCI-AML2 is a laboratory instrument designed for the analysis of liquid samples. It is capable of performing optical chromatography and ion analysis. The core function of the OCI-AML2 is to provide accurate and reliable measurement data for various liquid-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Hl 60, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Oci aml3, by American Type Culture Collection (2 mentions)
Oci aml3, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Mv4 11, by American Type Culture Collection (1 mentions)
Molm 13, by American Type Culture Collection (1 mentions)
Oci aml2, by American Type Culture Collection (1 mentions)
α mem medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hl 60, by American Type Culture Collection (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Hl 60, by Leibniz Institute DSMZ (1 mentions)
Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)
Aml 193, by Thermo Fisher Scientific (1 mentions)
Aml 193, by Leibniz Institute DSMZ (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
5 protocols using oci aml2
1
AML Cell Line Characterization
2
Cell Line Cultivation and Characterization
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Cell lines HS-5, KG-1a, HL-60, NB4, U937, and PBMC were obtained from American Type Culture Collection (ATCC), and OCI-AML2 was obtained from the German Collection of Microorganisms and Cell Cultures. KG-1a, HL-60, NB4, and U937 cells were cultured in RPMI-1640 medium (Gibco, USA), while HS-5 was cultured in DMEM (Gibco, USA). OCI-AML2 was cultured in MEM-α medium (Gibco, USA). The media used above contained 10% fetal bovine serum (FBS, USA) and 1% penicillin-streptomycin (Beyotime, Shanghai, China). The PBMC was not cultured after obtaining but was used directly for RNA extraction.
3
Culture conditions for human AML cell lines
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Human AML cell lines AML-193, KG-1, HL-60 and OCI-AML2 were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (Braunschweig, German). AML-193 cells were cultured in 90% Iscove’s Modified Dulbecco’s Medium (Gibco, USA) and 10% fetal bovine serum (Gibco, USA); KG-1 and HL-60 cells were cultured in 90% Roswell Park MEMorial Institute (RPMI) 1640 Medium (Gibco, USA) and 10% fetal bovine serum (Gibco, USA); OCI-AML2 were cultured in 90% Minimum Essential Medium (MEM) (Gibco, USA) and 10% fetal bovine serum (Gibco, USA).
4
Cell Culture Protocols for Cancer Research
5
Cell Line Cultivation and Characterization
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NB4, an APL-derived cell line, carrying the t(15;17) translocation was purchased from DSMZ (Braunschweig, Germany); U937, an histiocytic lymphoma-derived cell line; Mock and PR9 (a zinc-inducible PML/RARa model constructed from the U937 cell line) [34 (link)]; and HL-60, an AML FAB M2-derived cell line and Oci-AML2, an AML-M4-derived cell line carrying the DNMT3A R635W mutation (kindly provided by Emanuela Colombo, European Institute of Oncology, Milan, Italy) were grown at 37 °C in a humidified atmosphere of 5% CO2 in air in RPMI (Roswell Park Memorial Institute Medium) (GIBCO-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (GIBCO-BRL), 20 mM Hepes, 100 μg/mL penicillin and 100 μg/mL streptomycin (GIBCO-BRL).
The HEK293T, a human embryonal kidney cell line (kindly provided by Corinna Giorgi, European Brian Research Institute, Rome) was cultured as a monolayer in Dulbecco’s modified eagle medium containing 10% fetal bovine serum, 100 mg/mL penicillin and 100 mg/mL streptomycin.
Cells were tested regularly for mycoplasma contamination using a PCR kit (N-Garde EMK090020, Euroclone, Milan, Italy).
The HEK293T, a human embryonal kidney cell line (kindly provided by Corinna Giorgi, European Brian Research Institute, Rome) was cultured as a monolayer in Dulbecco’s modified eagle medium containing 10% fetal bovine serum, 100 mg/mL penicillin and 100 mg/mL streptomycin.
Cells were tested regularly for mycoplasma contamination using a PCR kit (N-Garde EMK090020, Euroclone, Milan, Italy).
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