Once mice reached adulthood (10–12 weeks), they were handled by experimenters for 2 min per day for three consecutive days prior to being run through a battery of behavioral assays (Figure 1 ): elevated plus maze (EPM), open field test (OFT), 3-D radial arm maze (3DR), 3-D open field test (3Doft), and two-period social interaction (2pSI), described in detail below. Each mouse was run in the OFT first, then once through each of the other assays, every 24 h (counterbalanced). Behavior was scored using an automated tracking system, EthoVision XT version 14 (Noldus, Leesburg, VA). On each test day, mice were acclimated to the behavioral testing room for at least 1 h prior to being tested. For each assay, lighting was adjusted to 12±2 lux.
Ethovision xt version 14
Manufactured by Noldus
EthoVision XT Version 14 is a video tracking and analysis software used for various behavioral research applications. It enables automatic tracking and analysis of animal movement and behavior in a range of experimental setups.
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Lab products found in correlation
4 protocols using ethovision xt version 14
1
Comprehensive Behavioral Phenotyping in Mice
2
Simultaneous Video Tracking and Respiratory Monitoring in Mice
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Whole-body plethysmography chambers with transparent bottom platforms were mounted on a box with a 1080p high-definition camera placed facing upward at the bottom of the structure. This camera allowed for simultaneous video recording of mouse movements during respiratory recordings. Mouse movements were tracked during both experimental days for the entire 3 h duration. Videos were recorded using Pinnacle Studio 24 MultiCam Capture software (Corel), resized, and exported to EthoVision XT Version 14 (Noldus) for movement analysis. Mouse velocity was calculated for minutes 5–10 and 50–55 postinjection in Microsoft Excel, and video data were aligned with respiratory recordings using video time stamps.
3
Locomotor Activity Profiling of Zebrafish Larvae
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A single zebrafish larva was placed in each well of a 96-well plate at 72 hpf (each group contained 18 zebrafish larvae) for behavioral assays. All behavioral tracking was performed in the DanioVision chamber, and the locomotor activity was monitored for 24 hours using the EthoVision XT Version 14 software (Noldus Information Technology). The tracking area inside the chamber was maintained at 28.5°C using a temperature control unit.
4
Automated Behavioral and Respiratory Monitoring in Mice
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Whole-body plethysmography chambers with transparent platforms were mounted on a box with an HD 1080 P high-definition camera placed at the bottom of the box. Mouse movements were tracked from below for the entire duration of the experiments. Videos were recorded using Pinnacle Studio 24 MultiCam Capture software (Corel, Ottawa, Ontario, Canada), resized using Pinnacle Studio 24, and exported to EthoVision XT Version 14 (Noldus, Wageningen, the Netherlands) for analysis of movement parameters. Mouse velocity (cm/s) and activity (% of pixel change) were quantified and exported to Microsoft Excel for analysis in parallel with the respiratory parameters during stimulation in freely behaving mice. Video data was aligned with plethysmography recordings using video time stamps. The behavioral state of the animal was defined before each stimulation using respiratory recording and video monitoring. The animal was considered in a calm state if respiratory rate was ≤125% of baseline respiratory rate, velocity (cm traveled per second) was ≤0.1 cm/s, and when activity (% of pixel changed in frame) was ≤0.4%. The animal was considered in an active state if respiratory rate was >125% of baseline respiratory rate, velocity was >0.1 cm/s, and activity was >0.4%.
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