Uas atg1 rnai

w¹¹¹⁸Canton-S (CS) were used as a “wild-type” strain throughout this manuscript. UAS-DN-S6K (6911), UAS-CA-S6K (6914), UAS-DN-AMPK (32112), UAS-CA-AMPK (32110), UAS-atg1-RNAi (44034), UAS-atg8a-RNAi (34340), and UAS-atg1 (51654) were obtained from the Bloomington Stock Center and outcrossed to w¹¹¹⁸Canton-S (CS) for at least 10 generations. tubulin-GAL4 flies were obtained from John Carlson, UAS-mCh-atg8a and UAS-GFP-Atg8a from Eric Baehrecke, and daughterless-Gene-Switch (daGS) from David W. Walker; all were outcrossed to w¹¹¹⁸Canton-S (CS) for at least 10 generations. period (per⁰¹) mutants, timeless-GAL4, and period-GAL4 were obtained from Jaga Giebultowicz and, because they were last outcrossed many years ago, were outcrossed to w¹¹¹⁸Canton-S (CS) for 12 generations in the last 2 years. UAS-gRNA, UAS-CAS9 lines were those utilized in our previous papers and were outcrossed to w¹¹¹⁸Canton-S (CS) for five generations.^{22 (link),39 (link)} Previously outcrossed cycle (cyc⁰¹) mutants were obtained from Sheyum Syed with a CS control strain. All experiments with multiple transgenes used flies that have undergone 12 generations of out-crossing into a w¹¹¹⁸Canton-S (CS) control and/or per⁰¹, w¹¹¹⁸Canton-S (CS) mutant background.