U937 cells were treated with PMA (10 ng/mL) and IL-4 (10 ng/mL) for TAMs induction 23 (link). TAMs were seeded onto a 0.4 μm pore Transwell chamber (Corning Life Sciences, MA, USA) to allow cytokines to cross over without cell-cell contact. PANC-1 and Mia PaCa-2 cells were seeded in 6-well plates and co-cultured with TAMs in 5% CO2 at 37 °C for 24 h. PCR and Western blot assays are described in the supplementary materials , and the sequences of primers and shRNA are shown in Table S1 .
0.4 μm pore transwell chamber
Manufactured by Corning
Sourced in United States
The 0.4 μm pore Transwell chamber is a lab equipment product designed for cell culture and migration studies. It features a permeable membrane with a pore size of 0.4 microns, allowing for the study of cell behavior and interactions across a defined barrier.
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Lab products found in correlation
2 protocols using 0.4 μm pore transwell chamber
1
Induction of Tumor-Associated Macrophages
2
Co-culture and Conditioned Medium Assay
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For the co-culture assay, we used a 0.4 μm pore Transwell chamber (Corning, USA). 4 × 105 colon cancer cells (SW480, HCT116) were plated in the bottom chamber of 6-well plates, while 4 × 105 Schwann cells (sNF96.2) were added into the upper Transwell insert. The cells were co-cultured for 48 hours and were used for subsequent experiments. To collect the conditioned medium (CM) of FHC, SW480, and HCT116 cells, 4 × 105 cells were plated in 6-well plates and cultured in DMEM/F12 or DMEM/high glucose for 48 h. The supernatant was collected and centrifuged it at 2000×g for 10 minutes to eliminate the cells and cell debris. All the CMs were used instantly or frozen at − 80 °C.
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