Direct 8 iso pgf2α elisa kit

Manufactured by Enzo Life Sciences

Sourced in United States, Switzerland

The Direct 8-iso-PGF2α ELISA kit is a laboratory tool used for the quantitative measurement of 8-iso-PGF2α, a biomarker of oxidative stress, in biological samples. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte in the sample.

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Close spelling variants of this product

PGF2α ELISA kit (6 citations) 8-iso-PGF2α ELISA kit (3 citations) PGF2α (3 citations)

4 protocols using direct 8 iso pgf2α elisa kit

Measurement of 8-iso-PGF2α in Serum

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To prepare the serum concentration of F2‐isoprostane, 8-Iso-Prostaglandin F2α (8-iso-PGF2α) was measured with a direct 8-iso-PGF2α ELISA kit (Enzo Life Sciences, Inc. Farmingdale, NY 11735) according to the manufacturer’s instructions. The absorbance was read at 405 nm with a UV spectrophotometer (Molecular Devices).

Lee J.S., Kim H.G., Lee D.S, & Son C.G. (2018). Oxidative Stress is a Convincing Contributor to Idiopathic Chronic Fatigue. Scientific Reports, 8, 12890.

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Liver Lipid Peroxidation Assay

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Livers were excised, weighed, and immediately frozen at −80 °C. The frozen tissues from each rat were homogenized in ice‐cold phosphate buffer (KCl 140 mM, phosphate 20 mM, pH 7.4) and centrifuged at 3000 rpm for 10 min. Protein concentration in the liver homogenates was determined using bovine albumin solution.21 LPO was determined by estimating the level of F2‐isoprostanes, a promising oxidative stress marker, which was detected in the liver using the commercially available Direct 8‐iso‐PGF2α ELISA kit (Enzo Life Sciences, Farmingdale, USA). A true reflection of both free and esterified isoprostane was measured following the manufacturer's instructions.

Bona S., Rodrigues G., Moreira A.J., Di Naso F.C., Dias A.S., Da Silveira T.R., Marroni C.A, & Marroni N.P. (2018). Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride. JGH Open: An Open Access Journal of Gastroenterology and Hepatology, 2(4), 117-123.

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Plasma and Heart Biomarker Analysis

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In plasma, assay kits were used to determine glycemia (Randox Laboratories, Crumlin, Antrim, UK), free fatty acids (NEFA kit, Roche, Mannheim, Germany), leptin (Rat leptin ELISA kit, Crystal Chem, Netherlands), LDL and HDL cholesterol (Abcam, ab65390) and 8-iso-PGF2α (Direct 8-iso-PGF2α ELISA kit, Enzo Life Science, Lausen, Switzerland).
In hearts, malondialdehyde (MDA) and mitochondrial citrate synthase (CS) activity were evaluated (MAK085-1KT, Sigma-Aldrich; CS0720, Sigma-Aldrich; Saint-Louis, MO, USA) after ischemia-reperfusion injury.

Fourny N., Lan C., Bernard M, & Desrois M. (2021). Male and Female Rats Have Different Physiological Response to High-Fat High-Sucrose Diet but Similar Myocardial Sensitivity to Ischemia-Reperfusion Injury. Nutrients, 13(9), 2914.

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Comprehensive Biomarker Profiling in Animal Model

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ELISAs were used to determine circulating and urinary ET-1 (ET-1 Quantikine ELISA Kit DET100, R&D systems), PGF_2α (circulating PGF_2α via 8-isoprostane ELISA Kit No. 516351, Cayman Chemical and urinary PGF_2α via Direct 8-iso-PGF_2α ELISA Kit ADI-900-091, Enzo Life Sciences), PGI₂ (6-keto-PGF_1α ELISA Kit ADI-900-004, Enzo Life Sciences) and TXA₂ (TXB₂ ELISA Kit ADI-900-002, Enzo Life Sciences) levels and the urinary excretion of albumin (Rat ELISA Ab108790, Abcam), neutrophil gelatinase-associated lipocalin (NGAL; Rat lipocalin-2 ELISA Kit Ab207925, Abcam), kidney injury molecule-1 (KIM-1) (Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit RKM100, R&D Systems), PGE₂ (Prostaglandin E₂ ELISA Kit No. 514010, Cayman Chemical) and its metabolite, PGE-M (Prostaglandin E Metabolite ELISA Kit No. 514531, Cayman Chemical).

Mirabito Colafella K.M., van Dorst D.C., Neuman R.I., Doorn L.V., Neves K.B., Montezano A.C., Garrelds I.M., van Veghel R., de Vries R., Uijl E., Clahsen-van Groningen M.C., Baelde H.J., van den Meiracker A.H., Touyz R.M., Visser W., Danser A.H, & Versmissen J. (2022). Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage. Clinical Science (London, England : 1979), 136(9), 675-694.

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