The human thyroid carcinoma cell lines, CAL-62 and SW579, were obtained from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. The immortalized normal human thyroid follicular epithelial cell line, Nthy-ori3-1, was purchased from CoBioer Biosciences Co., Ltd. (cat. no. CBP61205). HUVECs were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. HUVECs, CAL-62 and SW579 cells were cultured in DMEM supplemented with 1% penicillin/streptomycin and 10% FBS, at 37°C (5% CO2) in a humidified atmosphere. Nthy-ori3-1 cells were maintained in RPMI-1640 with 10% FBS and 1% penicillin/streptomycin.
Huvecs
Manufactured by Shanghai Zhong Qiao Xin Zhou Biotechnology
Sourced in China
HUVECs are primary human umbilical vein endothelial cells. They are a key tool in cell biology research, particularly for the study of vascular function and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
Hyclone, by GE Healthcare (2 mentions)
Endothelial growth factor, by Shanghai Zhong Qiao Xin Zhou Biotechnology (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Nthy ori3 1, by Cobioer Biosciences (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
Zq100, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Hey a8, by Thermo Fisher Scientific (1 mentions)
Csp006, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Heya8, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Huvecs, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Huvecs, by ScienCell (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Dmem basal medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
M199 complete medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
16 protocols using huvecs
1
Culturing Human Thyroid Cell Lines
2
Cell Culture of OSCC and HUVEC Lines
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Human CAL 27 and SCC-9 OSCC cell lines were obtained from the American Type Culture Collection (ATCC, USA). Human umbilical vein endothelial cells (HUVECs) were from Shanghai Zhong Qiao Xin Zhou Biotechnology (Shanghai, China). CAL 27 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Cat. A4,192,101, USA) containing 10% fetal bovine serum (FBS) (Gibco, Cat. 16,000,044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Cat. 10,378,016). SCC-9 cells were cultured in DMEM:F-12 Medium (DMEM-F12) (ATCC, Cat. 30-2006) supplemented with 10% FBS, 1% penicillin-streptomycin and 400 ng/mL hydrocortisone (Solarbio, Cat. G8450, China). HUVECs were maintained in Endothelial Cell Medium (Shanghai Zhong Qiao Xin Zhou Biotechnology, Cat. ZQ-1304) containing 5% FBS (Shanghai Zhong Qiao Xin Zhou Biotechnology, Cat. ZQ-1304), 1% Penicillin/Streptomycin Solution (Shanghai Zhong Qiao Xin Zhou Biotechnology, Cat. ZQ-1304) and 1% Endothelial Cell Growth Supplement (Shanghai Zhong Qiao Xin Zhou Biotechnology, Cat. ZQ-1304).
3
HUVEC Cell Culture Protocol
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HUVECs were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China) and cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin in humidified air containing 5% CO2 at 37°C.
4
HUVEC Culture Protocol
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HUVECs were purchased from Zhong Qiao Xin Zhou Biotechnology Co. Ltd (Shanghai, China). HUVECs were cultured in RPMI 1640 Medium (HyClone, Logan City, UT, USA) containing 10%FBS.
5
Transfection and Overexpression of ANGPTL4 and ESM1 in OC and HUVEC Cells
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OC cells (Hey-A8, and SKOV3, CVCL_0532) and human umbilical vein endothelial cells (HUVECs) were purchased from ATCC (Manassas, USA). These cells were cultured in McCoy’s 5 A (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd, ZQ-1000) (SKOV3) or DMEM (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd, ZQ-100) (HUVECs and Hey-A8) with 10% FBS (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd, ZQ500-A) and 1% PS (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd, CSP006). ANGPTL4 shRNA, ANGPTL4 OE plasmid, and ESM1 OE plasmid were purchased from HonorGene (Changsha, China). For cell transfection, these shRNAs or plasmids were transfected into the Hey-A8 and SKOV3 cell lines with Lipofectamine® 3000 (Thermo Fisher Scientific, Inc.) according to the manufacturer’s instructions.
6
Biocompatibility and Angiogenic Potential of HUVECs and HDFs
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Human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) were used for cell experiments. HUVECs were used to evaluate the biocompatibility, cell migration, and angiogenesis while HDF cells were used for biocompatibility evaluation and cell migration. HUVECs and HDFs were purchased from Zhong Qiao Xin Zhou Biotechnology Co,Ltd. (Shanghai, China). HUVECs were cultured with endothelial cell medium (ECM, Sciencell, USA). When the HUVECs were cultured to passage 3–5, they were used for cell experiments. HDF cells were cultured with dulbecco's modified eagle medium (DMEM, Gibco, USA) containing 10% FBS and 1% P/S. The HDF cells were used at passaged 7–10 for experiments.
7
Culturing Human Osteosarcoma and Endothelial Cells
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The human osteosarcoma HOS and MG63 cell lines were purchased from the Shanghai Zhong Qiao Xin Zhou Biotechnology Company Limited. The cells were cultured with RPMI 1640 containing 10% FBS. Human umbilical vein endothelial cells (HUVECs) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China). HUVECs were cultured in endothelial cell medium containing 10% FBS, endothelial cell growth supplement and 1% double antibiotics [18] . Both cell types were maintained in an incubator at 37 °C and 5% CO 2 . HOS cells in the logarithmic growth phase and HUVECs at passages three through six were used in the studies.
8
Scaffold Extract Preparation for Cell Culture
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Mouse macrophage cell line RAW 264.7 (Cell Research, Shanghai, China), mouse BMSCs (CRL-12424, ATCC, USA), and HUVECs (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd) were cultured in Dulbecco’s Modified Eagle Media (DMEM basal medium, Gibco, USA). Media were supplemented with penicillin/streptomycin (P/S) (1%, Life Technologies Corporation, USA) and 10% fetal bovine serum (FBS, Biological Industries, Israel). Cells were incubated at 37°C and 5% CO2 atmosphere.
In accordance with the guidelines of the International Organization for Standardization (ISO 10993-12), sterile scaffolds were submerged in serum-free DMEM at a ratio of 0.1 g/mL to obtain the scaffold extract (SE). The supernatant was collected, aseptically filtered, and kept at 4°C after being incubated at 37°C for 24 h. About 10% PBS and 1% P/S were then added.
In accordance with the guidelines of the International Organization for Standardization (ISO 10993-12), sterile scaffolds were submerged in serum-free DMEM at a ratio of 0.1 g/mL to obtain the scaffold extract (SE). The supernatant was collected, aseptically filtered, and kept at 4°C after being incubated at 37°C for 24 h. About 10% PBS and 1% P/S were then added.
9
Osteosarcoma and Endothelial Cell Cultivation
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The human osteosarcoma HOS and MG63 cell lines were purchased from the Shanghai Zhong Qiao Xin Zhou Biotechnology Company Limited. The cells were cultured with RPMI 1640 containing 10% FBS. Human umbilical vein endothelial cells (HUVECs) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China). HUVECs were cultured in endothelial cell medium containing 10% FBS, endothelial cell growth supplement and 1% double antibiotics (18 (link)). Both cell types were maintained in an incubator at 37°C and 5% CO2. HOS cells in the logarithmic growth phase and HUVECs at passages three through six were used in the studies.
10
Culturing Human Endothelial and Monocytic Cells
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Human umbilical vein endothelial cells (HUVECs) were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China), which was cultured in high-glucose Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37°C in an incubator containing 5% CO2. Human acute monocytic leukemia cell line (THP-1) was preserved in molecular biology laboratory, School of Traditional Chinese Medicine, Southern Medical University, which was cultured in RPMI 1640 medium with 10% FBS and 1% penicillin-streptomycin. All other conditions were the same as for HUVECs. The experiment cells were from the 4 to 10 passages.
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