Peroxidase affinipure goat anti human igg h l

Manufactured by Jackson ImmunoResearch

Sourced in China

Peroxidase AffiniPure goat anti-human IgG (H + L) is a secondary antibody conjugated with horseradish peroxidase. It is designed to bind to human immunoglobulin G (IgG) antibodies, including both the heavy and light chains.

Automatically generated - may contain errors

Lab products found in correlation

Versamax tunable microplate reader, by Molecular Devices (2 mentions) S1599, by Agilent Technologies (1 mentions) 96 well elisa plate, by Corning (1 mentions) Multiskan spectrum, by Thermo Fisher Scientific (1 mentions) Tween 20, by Merck Group (1 mentions) Peroxidase affinipure goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Antgene (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Bolt lds sample buffer, by Thermo Fisher Scientific (1 mentions) Azure c600 imaging system, by Azure Biosystems (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Peroxidase affinipure goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions)

6 protocols using peroxidase affinipure goat anti human igg h l

ELISA for Detecting PCNA-NKp44 Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 96-well ELISA plate (Costar) was coated overnight at 4°C with
0.1μM of the recombinant human PCNA (His and MBP tagged) and 0.1μM
of His-DJ1 (Human, negative control, a kind gift from Dr. Dan Levy, BGU, Israel)
in PBS. After washing, the plate was blocked with 2.5% skimmed milk in PBST
(PBS+0.05% TWEEN-20 [Sigma, P1379]) for 1h at 37°C followed by washing
and incubation with 2.5μg/ml of 14-25-9 and purified mouse IgG1κ
isotype control (BioLegend, 401402). Detection was done using mouse IgG
HRP-linked whole Ab (from Sheep) (GE Healthcare, NA931) (1:1000 final
dilutions). Following washing, TMB (DAKO, S1599) was added. Optical density
(O.D.) was measured at 650 nm (Thermo Electron Multiskan Spectrum).
In another setup, ELISA plates were coated overnight at 4°C with
0.1μM of the recombinant human PCNA (MBP tagged); blocking was done as
above. After blocking the plates were washed and incubated with 10 to
0.65μg/ml of 14-25-9 and PC10, 2.5μg/ml of mouse IgG1 and 0.25%
skimmed milk in PBST as control for 1h at 37°C and then NKp44-Ig (2
μg/ml, final concentration) was added without washing for 1h at
37°C. NKp44-Ig binding to PCNA was detected using peroxidase AffiniPure
goat anti-human IgG (H+L) (Jackson ImmunoResearch Laboratories,
Inc.) (1:1000 final dilution). Following washing, TMB
(100μl/well DAKO, S1599) was added. O.D. was read at 650 nm (Thermo
Electron Multiskan Spectrum).

Kundu K., Ghosh S., Sarkar R., Edri A., Brusilovsky M., Gershoni-Yahalom O., Yossef R., Shemesh A., Soria J.C., Lazar V., Ben-Zion J., Campbell K.S., Elkabets M, & Porgador A. (2019). Inhibition of the NKp44-PCNA Immune Checkpoint Using a mAb to PCNA. Cancer immunology research, 7(7), 1120-1134.

+ Open protocol

+ Expand

Quantifying Antibody Binding to Viral Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ELISA plates were coated with 0.5 μg/ml RBD-His, S-His, or EpEX-His protein in 0.1 M NaHCO₃ (pH 8.6) buffer at 4°C overnight, followed by blocking with PBS containing 1% bovine serum albumin (BSA) at RT for 2 h. After blocking, the wells were washed twice with PBS; the plates were then stored at -20°C.
The protein contents of the culture supernatants from hybridoma or antibodies were quantified by the BCA assay and serially diluted with 1% BSA in PBS. Then, 50 μl supernatant or antibody was added into each well, and the plate was incubated for 1 h at room temperature. The plates were washed with PBS containing 0.1% Tween-20 (PBST_0.1) three times and then incubated for 1 h with Peroxidase AffiniPure Goat Anti-mouse IgG (H+L) (Jackson ImmunoResearch) or Peroxidase AffiniPure Goat Anti-human IgG (H+L) (Jackson ImmunoResearch) (1:5000 dilution), as appropriate. After three washes with PBST_0.1, signal was produced using 3,3’5,5’-Tetramethylbenzidine (TMB) color development (TMBW-1000-01, SURMODICS). The reaction was stopped with 3 N HCl, and absorbance was measured at 450 nm by ELISA reader (Versa Max Tunable Microplate Reader; Molecular Devices).

Su S.C., Yang T.J., Yu P.Y., Liang K.H., Chen W.Y., Yang C.W., Lin H.T., Wang M.J., Lu R.M., Tso H.C., Chung M.J., Hsieh T.Y., Chang Y.L., Lin S.C., Hsu F.Y., Ke F.Y., Wu Y.H., Hwang Y.C., Liu I.J., Liang J.J., Liao C.C., Ko H.Y., Sun C.P., Wu P.Y., Jan J.T., Chang Y.C., Lin Y.L., Tao M.H., Hsu S.T, & Wu H.C. (2021). Structure-guided antibody cocktail for prevention and treatment of COVID-19. PLoS Pathogens, 17(10), e1009704.

+ Open protocol

+ Expand

Western Blot Analysis of CD4 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sample preparation, loading, membrane transfer, and blocking were performed as described above. Membranes were blocked in 5% milk in PBS and then incubated with the recombinant anti-CD4 antibody (Abcam, #ab133616) diluted 1:1,000 in 2% milk in PBS overnight at 4°C. Blots were then washed in 0.1% TBST and incubated with Peroxidase AffiniPure goat anti-human IgG (H + L) (Jackson ImmunoResearch Laboratories, #109-035-088) diluted 1:10,000 in 5% BSA in TBS for 2 h at room temperature. Blots were washed, imaged, and visualized as described above.

Vamva E., Ozog S., Leaman D.P., Yu-Hong Cheng R., Irons N.J., Ott A., Stoffers C., Khan I., Goebrecht G.K., Gardner M.R., Farzan M., Rawlings D.J., Zwick M.B., James R.G, & Torbett B.E. (2023). A lentiviral vector B cell gene therapy platform for the delivery of the anti-HIV-1 eCD4-Ig-knob-in-hole-reversed immunoadhesin. Molecular Therapy. Methods & Clinical Development, 28, 366-384.

+ Open protocol

+ Expand

Monoclonal Antibodies for ASFV Detection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The monoclonal antibodies against ASFV p72, p30, and p54 [23 (link)] were prepared in our laboratory for western blotting and immunofluorescence assay (IFA). Antibodies and dyes were purchased, including DAPI (Beyotime, Shanghai, China), Alexa Fluor®488 Donkey anti Mouse IgG (antGene, Wuhan, China), Peroxidase-AffiniPure Goat Anti-Human IgG (H + L) (Jackson ImmunoResearch Inc, West Grove, PA, USA), HRP Goat Anti-Mouse IgG (ABclone, Wuhan, China). Primers and probes for gene amplification were synthesized by Sangon Biotech (Sangon, Shanghai, China).

Cao H., Zhang M., Liao Z., Li D., He X., Ma H., Li P., Yu X., Peng G., Xie S., He Q, & Li W. (2024). A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus. Veterinary Research, 55, 42.

+ Open protocol

+ Expand

Monitoring eCD4-Ig Dimerization in Transduced B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatant from transduced B cells was harvested 5 days, 8 days, and 11 days post transduction to study eCD4-Ig expression and dimerization status at the different stages of B cell differentiation. Equal volumes of eCD4-Ig-containing cell supernatants were mixed with cold 4× Bolt LDS Sample Buffer (Thermo Fisher) and directly loaded on a 4%–12% Bis-Tris Bolt Precast Gel (Thermo Fisher) in cold 1× Bolt MES SDS running under non-reducing conditions to preserve protein dimers. The gel was then transferred to an activated PVDF membrane using the Trans-Blot turbo transfer system (Bio-Rad). Membranes were blocked in 5% milk in PBS for 2 h at room temperature and were subsequently incubated with Peroxidase AffiniPure goat anti-human IgG (H + L) (Jackson ImmunoResearch Laboratories, #109-035-088) diluted 1:10,000 in 2% milk in PBS for 2 h at room temperature. Blots were washed in 0.1% Tween containing 1× Tris-buffered saline (TBST) and imaged using ECL Western Blotting Substrate. Results were visualized on the Azure c600 imaging system (Azure Biosystems).

+ Open protocol

+ Expand

SARS-CoV-2 Variant Spike Protein ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

ELISA plates were coated with 0.5 μg/ml SARS-CoV-2 variant S-His protein or EpEX-His protein (negative control) in 0.1 M NaHCO₃ (pH 8.6) buffer at 4 °C overnight, followed by blocking with PBS containing 1% bovine serum albumin (BSA) at RT for 2 h. After blocking, the wells were washed twice with PBS; then, the plates were stored at − 20 °C. RBD-chAb or rabbit anti-His Ab was added at a concentration of 30 ng/ml in each well, and the plate was incubated for 1 h at room temperature. The plates were washed with PBS containing 0.1% Tween-20 (PBST_0.1) three times and then incubated for 1 h with Peroxidase AffiniPure Goat Anti-human IgG (H + L) (Jackson ImmunoResearch) (1:4000 dilution) or Peroxidase AffiniPure Goat Anti-rabbit IgG (H + L) (Jackson ImmunoResearch) (1:10,000 dilution), as appropriate. After three washes with PBST_0.1, signal was produced using 3,3′5,5'-Tetramethylbenzidine (TMB) color development (TMBW-1000–01, SURMODICS). The reaction was stopped with 3 N HCl, and absorbance was measured at 450 nm by ELISA reader (Versa Max Tunable Microplate Reader; Molecular Devices).

Liang K.H., Chiang P.Y., Ko S.H., Chou Y.C., Lu R.M., Lin H.T., Chen W.Y., Lin Y.L., Tao M.H., Jan J.T, & Wu H.C. (2021). Antibody cocktail effective against variants of SARS-CoV-2. Journal of Biomedical Science, 28, 80.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!