2 thiobarbituric acid

Acrylamide, N,N-methylene bisacrylamide, glycine and tris base were purchased from Jintai Hongda Biotechnology Co., Ltd. (Beijing, China). 2-Thiobarbituric acid, dithiothreitol, thiourea, Coomassie Brilliant Blue R-250, sodium dodecyl sulphate (SDS), bromophenol blue and 2,4-dinitrophenylhydrazine were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). N,N,N,N-tetramethylethylenediamine and β-mercaptoethanol were acquired from Macleans Biochemical Technology Co., Ltd. (Shanghai, China). Coomassie Brilliant Blue kit was provided by Nanjing Jiancheng Institute of Bioengineering (Jiangsu, China). 1,1,3,3-Tetraethoxypropane and chromatography grade formic acid were supplied by Sigma-Aldrich Co., Ltd. (MO, USA). Mass spectrometry-grade trypsin, acetonitrile and water were obtained from Thermo Fisher Scientific (MA, USA). All other chemical reagents were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Plate count agar (PCA) and iron agar medium were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, Shandong, China). Magnesium oxide, boric acid, sodium chloride, and 2-thiobarbituric acid were purchased from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). Other agents of analytic grade were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China).