Dimethyl sulfoxide d6 dmso d6

CSP7 acetate (purity > 95%) and CSP7 trifluoroacetate (purity > 95%) were kindly provided by Lung Therapeutics Inc. (Austin, TX, USA). D-Mannitol, D-trehalose, deuterium oxide, and dimethyl sulfoxide-d₆ (DMSO-D₆) were purchased from Acros Organic (Morris, NJ, USA). Lactose monohydrate, sodium chloride, disodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, ammonium hydroxide solution (28% v/v in water), and acetonitrile (HPLC grade) were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Dulbecco’s phosphate buffered saline pH 7.4 (DPBS), which is composed of 2.7 mM potassium chloride, 1.5 mM monopotassium phosphate, 136.9 mM sodium chloride, 8.1 mM disodium phosphate heptahydrate, was purchased from Lonza (Morristown, NJ, USA). Tris(hydroxymethyl)aminomethane, hydrochloric acid solution (1.0 M), Hydranal^®-Coulomat AG and Hydranal^®-Coulomat CG were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sterile water injection USP (SWI) was purchased from Forty Aces Pharmacy (Austin, TX, USA).

Analytical grade ferric chloride, ferrous chloride, ammonia solution (25 wt %), thiophene, 4-hydroxybenzaldehyde, acetonitrile, potassium permanganate, 4-aminophenol, 3-bromothiophene, 1,6-dibromohexane, N-bromosuccinimide, acetic acid, sodium hydrogen bicarbonate, potassium iodide, potassium carbonate, tetrahydrofuran, methanol, hydrochloric acid, acetone and ethyl acetate were purchased from Merck (Darmstadt, Germany). acetone was procured from Fisher Scientific (Loughborough, UK). thiophene carboxaldehyde, polyvinyl alcohol and n-butyllithium (2.0 M in cyclohexane) were obtained from Sigma Aldrich (Milwaukee, WI, USA). Magnesium sulfate anhydrous, ethanol denatured and hexane were received from J. Kollins (Parkwood, Australia), while dimethyl sulfoxide-d₆ (DMSO-d₆) and phthalate esters were purchased from Acros Organics (Geel, Belgium). Ultrapure water was prepared by a model Aqua Max-Ultra ultra-pure water purification system (Zef Scientific Inc., San Diego, CA, USA). Stock solutions of 1000 mg·L⁻¹ of standards were prepared by dissolving appropriate amounts of compounds in methanol, which remain stable for three months if stored in a refrigerator at 4 °C. Working standard solutions were prepared daily by diluting the stock standard solution to the required concentrations.

PPO (Mn 20,000 and Polydispersity ~2.5) was purchased from Polysciences, Inc. Chlorobenzene (ACS reagent, ≥99.5%), N-methyl-2-pyrrolidone (NMP, reagent grade), tetrahydrofuran (THF, ACS, >99%), diethyl ether (>99%), 1,2-dichloroethane (99.8%), allyl bromide (98%), allyl chloride (98%) and chloroform (99.8%) were purchased from Alfa Aesar (Thermo Fisher, Kandel, Germany). Diallymethylamine (97%) and piperidine (≥99%) were bought from ABCR GmbH (Karlsruhe, Germany). Dimethyl sulfoxide-d₆ (DMSO-d₆, 99.9%) was supplied from Acros Organics ((Thermo Fisher, Kandel, Germany). N-bromosuccinimide (NBS, 99%), 2,2′-azobis(2-methylpropionitrile) (AIBN, 98%), methanol (99.9%) and chloroform-d (CDCl₃-d, 99.9% D) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) was bought from Ciba Specialty Chemicals Inc (Basel, Switzerland). Cellophane™ 350PØØ was purchased from Futamura Chemical Co. Ltd. (Hamburg, Germany). Celgard^® membranes were kindly provided by Celgard, LLC (France). Zirfon^® and PBI^® were provided by AGFA Gevaert NV (Mortsel, Belgium) and Fumatech BWT GmbH (Bietigheim-Bissingen, Germany), respectively. All chemicals were used without further purification.