Anti patm s1981

The antibodies used were as follows: anti-Banf1 N-terminus (SAB1409950, Sigma-Aldrich, 1:1000 for WB and 1:500 for IF), anti-Banf1 C-terminus (PRS40170604, Sigma-Aldrich, 1:1000 for WB and 1:500 for IF), anti-Emerin (5430, Cell Signalling Technology, 1:500 for IF), anti-Flag M2 Antibody (F3165, Sigma-Aldrich, 1:1000 for WB and 1:300 for IF), anti-γ-Tubulin (T6557, Sigma-Aldrich, 1:2000 for WB), anti-H3 (4499, Cell Signaling Technology, 1:2000 for WB), anti-β-actin (612656, BD Biosciences, 1:2000 for WB), anti-P-ATM S1981 (5883, Cell Signaling Technology 1:1000), anti-Mre11 (HPA002691, Sigma-Aldrich, 1:1000 for WB), anti-MDC1 (PLA0016, Sigma-Aldrich, 1:1000 for IF), anti-γ-H2AX (ab26350, abcam, 1:1000 for WB; 9718, Cell Signalling Technology, 1:1000 for IF, ab81299, abcam for ChIP), anti-p-DNA-PK S2056 (Abcam, ab124918, 1:1000 wb), anti-DNA-PKcs (12311, Cell Signaling Technology 1:1000 for western blot and ab70205 1:200 for IF), anti-Ligase IV (ab193353, Abcam, 1:1000), anti-Ku70 (ab92450, Abcam, 1:1000) and anti-nucleolin (14574, Abcam, 1:1000). Fluorescent secondary antibodies used were: Donkey anti-Mouse 800 nm (LiCor; IRDye 800CW 926–32212, 1:5000 for WB), Donkey anti-Rabbit (LiCor; IRDye 680LT 926–28023, 1:5000 for WB) and Alexa Fluor 488 (Cat# A32766, Molecular Probes, 1:200 for IF) and 594 (Cat# A32754, Molecular Probes, 1:200 for IF).