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Cd14 buv395

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CD14-BUV395 is a fluorochrome-conjugated antibody that binds to the CD14 cell surface antigen. CD14 is a glycosylphosphatidylinositol-anchored protein expressed on the surface of monocytes, macrophages, and neutrophils. The BUV395 fluorochrome is a proprietary dye developed by BD Biosciences.

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Lab products found in correlation

Cd15 bv421, by BD (1 mentions) Cd36 percp, by Miltenyi Biotec (1 mentions) Cd16 bv480, by BD (1 mentions) Cd16b pe, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Brilliant stain buffer, by BD (1 mentions) Cd16 bv421, by BD (1 mentions) Cd45 bv786, by BD (1 mentions) Hla dr percp, by BD (1 mentions) Cd4 pacific blue, by BD (1 mentions) Cd3 alexa fluor 700, by BD (1 mentions) Cd38 pe, by BD (1 mentions) Cd14 apc cy7, by BD (1 mentions) Cd8 pe cy7, by BD (1 mentions) Fluorescein isothiocyanate (fitc), by BD (1 mentions) Cd123 pe cy7, by BioLegend (1 mentions) 4 laser lsr 2, by BD (1 mentions) Cd16 bv 510, by BioLegend (1 mentions) Ccr2 pe, by BioLegend (1 mentions)

Spelling variants of this product

CD14-BUV395 (clone M5E2, (2 citations)

4 protocols using cd14 buv395

1

Monitoring Monocyte-Macrophage Differentiation and Neutrophil Development

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Monocytes were grown for 15 days in order to check their developing to macrophages by specific antibodies: CD14-BUV395 (BD-563561), CD15-BV421 (BD-740086) and CD36-PerCP (Miltenyi-130 095 480). Simultaneously, the fusion event was followed-up with the above markers during the development process of both monocytes (d0 to d5) and macrophages (d10 to d15).
Neutrophils were isolated from healthy donors using dextran protocol,25 (link) and their development and fusion affinity were followed-up for 5 days by specific markers: CD14-BUV395, CD15-BV421, CD16-BV480 (BD-566108), CD16b-PE (BD-550868), CD36-PerCP and CD66b-PE (Miltenyi-130 104 396).
Aguirre L.A., Montalbán-Hernández K., Avendaño-Ortiz J., Marín E., Lozano R., Toledano V., Sánchez-Maroto L., Terrón V., Valentín J., Pulido E., Casalvilla J.C., Rubio C., Diekhorst L., Laso-García F., del Fresno C., Collazo-Lorduy A., Jiménez-Munarriz B., Gómez-Campelo P., Llanos-González E., Fernández-Velasco M., Rodríguez-Antolín C., Pérez de Diego R., Cantero-Cid R., Hernádez-Jimenez E., Álvarez E., Rosas R., dies López-Ayllón B., de Castro J., Wculek S.K., Cubillos-Zapata C., Ibáñez de Cáceres I., Díaz-Agero P., Gutiérrez Fernández M., Paz de Miguel M., Sancho D., Schulte L., Perona R., Belda-Iniesta C., Boscá L, & López-Collazo E. (2020). Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells. Oncoimmunology, 9(1), 1773204.
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2

Monocyte Subsets Analysis by Flow Cytometry

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Monocyte subsets at fasting and postprandial time points were analyzed using 100 µl of whole blood collected into EDTA-treated tubes mixed with pre-titrated volumes of the following antibodies in BD Brilliant stain buffer (catalog # 563,794 BD Biosciences): CD45-BV786 (catalog # 563,716 BD Biosciences), CD91-PE (catalog # 550,497 BD Biosciences), CD14-BUV395 (catalog # 563,561 BD Biosciences), and CD16-BV421 (catalog # 562,878 BD Biosciences). Following a 20-min incubation at room temperature 1X BD FACS Lysing Solution (catalog# 349,202 BD Biosciences) was added to whole blood/antibody mixture and incubated at room temperature for an additional 10 min. Cells were washed twice with cold wash/stain buffer (containing 0.1% BSA (w/v), 0.05% NaN3 (w/v) in PBS) then analyzed using an LSR Fortessa flow cytometer (BD Biosciences) configured with blue (488 nm), red (640 nm), violet (405 nm) and UV lasers (355 nm). Data were collected using FACSDiva and analyzed using FlowJo version 10.6.1 software (BD Biosciences).
Snodgrass R.G., Jiang X, & Stephensen C.B. (2022). Monocyte subsets display age-dependent alterations at fasting and undergo non-age-dependent changes following consumption of a meal. Immunity & Ageing : I & A, 19, 41.
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3

Multiparameter Flow Cytometry of T Cell Activation

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CD4 and CD8 T cell activation was measured by flow cytometry on a 4-laser LSR II (BD Biosciences). T cell populations were defined as CD14− CD3+ and either CD4+ or CD8+. Activation was quantified on the basis of the percentage of CD4+ or CD8+ cells that were double positive for CD38 and HLA-DR. The T cell activation panel included the following Abs: CD14 APC-Cy7, HLA-DR–PerCP, CD4 Pacific Blue, CD3 Alexa Fluor 700, CD8 PE-Cy7, and CD38 PE (all from BD Biosciences). The monocyte panel consisted of CD3, CD19, and CD56 — all on FITC (BD) and CD14 BUV 395, CD16 BV510, CCR2 PE, CX3CR1 APC, CD11c PEcf594, CD80 BV421, CD86 BV605, HLA-DR BV785, CD123 PECy7, and eFluor 780 fixable viability dye (BioLegend). Data were acquired on a 4 laser LSR II and analyzed with FlowJo software. Additional details are provided in Supplemental Table 2.
Scully E.P., Lockhart A., Garcia-Beltran W., Palmer C.D., Musante C., Rosenberg E., Allen T.M., Chang J.J., Bosch R.J, & Altfeld M. (2016). Innate immune reconstitution with suppression of HIV-1. JCI Insight, 1(3), e85433.
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4

Differentiation and EBOV Infection in THP-1 Cells

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THP-1 cells were plated at 1x106 cells/ml in 24-well plates in medium alone or medium containing CLI-095 (100 ng/ml) for 1 h. Thereafter LPS (500 ng/ml), EBOV (no GFP), EBOV GP beads or HPIV3/ΔF-HN/EboGP were added at MOI 3 PFU/cell and cells were incubated for 24 h or 96 h. To analyze markers of differentiation, cells were harvested, stained with antibodies specific for CD14-BUV395 (BD Biosciences #563561) and CD11b-FITC (BD Biosciences #562793), permeabilized, fixed and stained with antibodies specific for CD68-PE/Cy7 (BD Biosciences #565595). To analyze susceptibility to infection, following stimulation cells were centrifuged for 5 min at 250 g and supernatants were removed, cells were infected with EBOV-GFP at MOI of 3 PFU/cell, and incubated for an additional 48 h. CLI-095 (100 ng/ml) was added 1h prior cells were cultured with conditions. Cells were harvested, fixed and analyzed and analyzed using for markers of differentiation or GFP by FACS Fortessa flow cytometer (BD Biosciences).
Iampietro M., Younan P., Nishida A., Dutta M., Lubaki N.M., Santos R.I., Koup R.A., Katze M.G, & Bukreyev A. (2017). Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathogens, 13(5), e1006397.
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