Molecular weights (Mn and Mw) and polydispersity indices (PDIs) of all polymers were measured using a gel permeation chromatography (Viscotek) instrument equipped with triple detectors, including a refractive index detector, a viscometer detector, and a light scattering detector. The morphologies of the micelles were studied by transmission electron microscopy (TEM, FEI Tecnai G2 F30 TWIN 300 kV, E.A. Fischione Instruments, Inc.) and dynamic light scattering (DLS, ZetaSizer Nano ZS90, Malvern Instruments). The AF loading level (i.e., the weight percentage of AF in AF-loaded micelles) was determined by UV–vis spectrometry (Varian Cary 300 Bio UV–vis spectrophotometer, Agilent Technologies, Santa Clara, CA) at 357 nm. The temperature-dependent phase transition of CuS-based micelles was determined by measuring their optical transmittances in an aqueous solution (3 mg/mL) at 670 nm at various temperatures using a UV–vis spectrophotometer.38
Varian cary 300 bio uv vis spectrophotometer
Manufactured by Agilent Technologies
The Varian Cary 300 Bio UV–vis spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light in the ultraviolet and visible spectrum. It is capable of performing a wide range of spectroscopic analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using varian cary 300 bio uv vis spectrophotometer
1
Characterization of CuS-based Micelles
2
Spectrophotometric Enzyme Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzyme activities were determined according to a modified absorption assay [44] (link), [45] (link). Protein concentrations were 0.25 mg/ml and 0.1 mg/ml for monophenolase and diphenolase activity experiments, respectively. Protein monophenolase and diphenol oxidase activities were measured using L-tyrosine and L-DOPA (Sigma-Aldrich, MO) as substrates, respectively. The reaction mixture containing 0.2 mM L-tyrosine or 3 mM L-DOPA in 50 mM sodium phosphate buffer, pH 7.5 was incubated for 30 min at 37°C and monitored by measuring the dopachrome formation at 490 nm (εdopachrome = 3520 M−1 cm−1) using a Varian Cary 300 Bio UV-Vis Spectrophotometer (Agilent Technologies, CA). For monophenol oxidase activity, additional 50 µM L-DOPA was added to eliminate a lag period [46] (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!