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Nickel coated 96 well plates

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Nickel coated 96 well plates are a type of laboratory equipment used for various assays and experiments. These plates have a nickel coating on the surface of the wells, which can provide specific properties or functionalities required for certain applications. The core function of these plates is to serve as a platform for conducting various biochemical, cell-based, or other types of analyses in a standardized 96-well format.

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Lab products found in correlation

Opti mem 1, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Formaldehyde, by Merck Group (1 mentions) Turkey red blood cells trbcs, by Charles River Laboratories (1 mentions) 0.45 μm polyvinylidene difluoride pvdf membrane, by GE Healthcare (1 mentions) Novex 4 12 tris glycinesds page gels, by Thermo Fisher Scientific (1 mentions) Simple blue stain, by Thermo Fisher Scientific (1 mentions) Specific pathogen free eggs, by Charles River Laboratories (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) β propiolactone, by Merck Group (1 mentions) Anti rabbit igg hrp linked secondary antibody, by GE Healthcare (1 mentions) O phenylenediamine dihydrochloride opd, by Merck Group (1 mentions)

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96-well plates (1169 citations) Nickel-coated plates (4 citations)

4 protocols using nickel coated 96 well plates

1

Trimer-Antibody Binding Assay

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HisTagged trimers were coated at 2 μg/mL in PBS onto nickel coated 96 well plates (Thermo) for one hour at 25°C. Plates were washed and then probed with serial dilutions of HIV-1 monoclonal antibody for one hour at 25°C. This process was repeated using an anti-Fc/HRP conjugate to detect trimer-bound antibodies. Antigen-antibody complexes were detected by incubating with 100 μL of enzyme substrate for five minutes and then the reaction was stopped with 25 μL of 1 M HCl. Absorbance was read at 450 nm.
Wibmer C.K., Gorman J., Ozorowski G., Bhiman J.N., Sheward D.J., Elliott D.H., Rouelle J., Smira A., Joyce M.G., Ndabambi N., Druz A., Asokan M., Burton D.R., Connors M., Abdool Karim S.S., Mascola J.R., Robinson J.E., Ward A.B., Williamson C., Kwong P.D., Morris L, & Moore P.L. (2017). Structure and Recognition of a Novel HIV-1 gp120-gp41 Interface Antibody that Caused MPER Exposure through Viral Escape. PLoS Pathogens, 13(1), e1006074.
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2

Influenza Virus Hemagglutinin and Neuraminidase Assay

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Dulbecco’s Modified Eagles Medium (DMEM), fetal bovine serum (FBS), L-glutamine, penicillin/streptomycin (P/S), Opti-MEM I (OMEM), anti-goat IgG HRP-linked secondary antibody, Simple Blue Stain, Novex 4–12% Tris-Glycine SDS-PAGE gels, dithiothreitol (DTT) Immulon-2HB 96-well plates, Nickel coated 96-well plates and Lipofectamine 2000 transfection reagent were all purchased from Thermo Fisher Scientific. Zanamivir and 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (MUNANA) were acquired from Moravek Inc and Cayman Chemicals, respectively. β-propiolactone, formaldehyde, o-Phenylenediamine dihydrochloride (OPD), and anti-mouse IgG HRP linked secondary were purchased from Sigma. Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF) eggs and turkey red blood cells (TRBCs) were purchased from Charles River Labs and the Poultry Diagnostic and Research Center (Athens, GA), respectively. The H1N1 A/Brisbane/02/2018 field isolate (WT) and CVV (IVR-190) were kindly provided by the WHO. Rabbit Antisera against NA was generated by Agrisera (Sweden) using NA-WSN residues 35–453 isolated from E. coli inclusion bodies [29 (link)]. Polyclonal goat antiserum against the H1N1 influenza virus A/Fort Monmouth/1/1947 (NR-3117) was obtained from BEI Resources, NIAID, NIH.
Gao J., Wan H., Li X., Rakic Martinez M., Klenow L., Gao Y., Ye Z, & Daniels R. (2021). Balancing the influenza neuraminidase and hemagglutinin responses by exchanging the vaccine virus backbone. PLoS Pathogens, 17(4), e1009171.
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3

ELISA to Quantify V1V2-Directed Antibodies

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96-well nickel coated plates (Thermo Fisher Scientific) were coated for 1 h at 100 μl/well with his-tagged BG505 SOSIP.664.DS.V1V2 constructs at 1 μg/ml, diluted in 1× PBS. The plates were then blocked at room temperature for 1 h using 200 μl/well of 5% skim milk in 1× PBS. After the plates were washed (wash buffer, 0.05% Tween 20 plus 1× PBS), they were incubated for 1 h at room temperature with 5-fold serially diluted V1V2-directed antibodies at concentrations ranging from 20 to μg/ml to 1 ng/ml in 1 × PBS plus 0.05% Tween 20. The plates were washed and then incubated for another hour with horseradish peroxidase (HRP)-conjugated anti-human IgG (1:5000) diluted in 0.2% Tween 20 plus 1× PBS. After a final wash, the plates were then developed using TMB peroxidase substrate for 20 min. The reaction was stopped with 140 mM HCl, and then the absorbance was measured at 450 nm. All incubations were at 100 μl/well at room temperature, except where noted otherwise. Experiments were run in triplicate.
Gorman J., Soto C., Yang M.M., Davenport T.M., Guttman M., Bailer R.T., Chambers M., Chuang G.Y., DeKosky B.J., Doria-Rose N.A., Druz A., Ernandes M.J., Georgiev I.S., Jarosinski M.C., Joyce M.G., Lemmin T.M., Leung S., Louder M.K., McDaniel J.R., Narpala S., Pancera M., Stuckey J., Wu X., Yang Y., Zhang B., Zhou T., Mullikin J.C., Baxa U., Georgiou G., McDermott A.B., Bonsignori M., Haynes B.F., Moore P.L., Morris L., Lee K.K., Shapiro L., Mascola J.R, & Kwong P.D. (2015). Structures of HIV-1-Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design. Nature structural & molecular biology, 23(1), 81-90.
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4

ELISA to Quantify V1V2-Directed Antibodies

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96-well nickel coated plates (Thermo Fisher Scientific) were coated for 1 h at 100 μl/well with his-tagged BG505 SOSIP.664.DS.V1V2 constructs at 1 μg/ml, diluted in 1× PBS. The plates were then blocked at room temperature for 1 h using 200 μl/well of 5% skim milk in 1× PBS. After the plates were washed (wash buffer, 0.05% Tween 20 plus 1× PBS), they were incubated for 1 h at room temperature with 5-fold serially diluted V1V2-directed antibodies at concentrations ranging from 20 to μg/ml to 1 ng/ml in 1 × PBS plus 0.05% Tween 20. The plates were washed and then incubated for another hour with horseradish peroxidase (HRP)-conjugated anti-human IgG (1:5000) diluted in 0.2% Tween 20 plus 1× PBS. After a final wash, the plates were then developed using TMB peroxidase substrate for 20 min. The reaction was stopped with 140 mM HCl, and then the absorbance was measured at 450 nm. All incubations were at 100 μl/well at room temperature, except where noted otherwise. Experiments were run in triplicate.
Gorman J., Soto C., Yang M.M., Davenport T.M., Guttman M., Bailer R.T., Chambers M., Chuang G.Y., DeKosky B.J., Doria-Rose N.A., Druz A., Ernandes M.J., Georgiev I.S., Jarosinski M.C., Joyce M.G., Lemmin T.M., Leung S., Louder M.K., McDaniel J.R., Narpala S., Pancera M., Stuckey J., Wu X., Yang Y., Zhang B., Zhou T., Mullikin J.C., Baxa U., Georgiou G., McDermott A.B., Bonsignori M., Haynes B.F., Moore P.L., Morris L., Lee K.K., Shapiro L., Mascola J.R, & Kwong P.D. (2015). Structures of HIV-1-Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design. Nature structural & molecular biology, 23(1), 81-90.
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