Two 96-position plate designs (plate 1 and 2) were constructed with the 31 strains, each randomized at six different positions (three positions per plate), leaving three empty positions as negative controls (Figure 1A , Supplementary Table 1 ). Then each plate was replicated on PCASL medium and on PCASL supplemented with 3% NaCl, 5% NaCl, or 3% KCl. Each plate and condition were produced in triplicate for a total of 24 monoculture plates. To assess the impact of microbial interactions, co-cultures were created by pinning plates 1 and 2 on top of each other, resulting in 93 pairs on the interaction plate. This design was replicated three times on each culture medium (PCASL as the controls and PCASL with 3% NaCl, 5% NaCl, or 3% KCl as the treatments). All plates were incubated at 20°C for 10 days. The automated platform was equipped with a camera (Canon EOS Rebel T7i) to capture images of OmniTray™ plates. The first images were taken after 24 h of incubation at 20°C and subsequently at regular intervals during the day for 9 days for a total of 18 time points.
Eos rebel t7i
Manufactured by Canon
Sourced in Japan
The Canon EOS Rebel T7i is a digital single-lens reflex (DSLR) camera. It features a 24.2-megapixel APS-C CMOS sensor and a DIGIC 7 image processor. The camera supports full HD 1080p video recording at up to 60 frames per second. It has a 3-inch touchscreen LCD display and an optical viewfinder. The EOS Rebel T7i is compatible with Canon's EF and EF-S lens lineup.
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Lab products found in correlation
9 protocols using eos rebel t7i
1
High-Throughput Microbial Interaction Screening
2
Alginate Bead Synthesis and Analysis
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Data are shown in the form mean ± standard error of the mean (SEM). Significance was chosen as p < 0.05. This was determined using both one- and two-way analysis of variance (ANOVA) along with the Student’s t-test. These analyses were performed utilizing Microsoft Excel’s data analysis software package.
For image analysis, ImageJ (Version 1.52o with the addition of plug-ins for colocalization (Colocalization Finder, Version 1.2, Institut de Biologie Moleculaire des Plantes, Strasbourg, France) was used to estimate the overlap coefficient between each layer of beads and to generate representative pictures. Also, it was used in the diameter measurements of the alginate beads that were synthesized. Images and videos in this experiment were taken with a stereomicroscope (Olympus SZ61, Olympus, Center Valley, PA, USA), a phase-contrast microscope (AMG EVOS FL Imaging System, Thermo Fisher Scientific, Waltham, MA, USA), and a Canon camera (Canon EOS Rebel T7i, Canon, Tokyo, Japan).
For image analysis, ImageJ (Version 1.52o with the addition of plug-ins for colocalization (Colocalization Finder, Version 1.2, Institut de Biologie Moleculaire des Plantes, Strasbourg, France) was used to estimate the overlap coefficient between each layer of beads and to generate representative pictures. Also, it was used in the diameter measurements of the alginate beads that were synthesized. Images and videos in this experiment were taken with a stereomicroscope (Olympus SZ61, Olympus, Center Valley, PA, USA), a phase-contrast microscope (AMG EVOS FL Imaging System, Thermo Fisher Scientific, Waltham, MA, USA), and a Canon camera (Canon EOS Rebel T7i, Canon, Tokyo, Japan).
3
Colony Photography of Bacterial Cultures
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After 6 days of culture at 25°C on M9 or M63-1% agar (same conditions as for Congo red), colonies were photographed using a Canon EOS Rebel T7i camera equipped with a Canon Macro Lens EF-S 35 mm lens. Photographs were taken under soft white lighting on a black-felted copy stand.
4
Microfluidic Flow Visualization Technique
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To visualize microfluidic flows,
the fully packaged ND probe (Figure 4 a) was connected to external pressure-controlled pumps
(Flow-EZ Module, LU-FEZ-7000, and LU-FEZ-N800, Fluigent) for push
(P2 inFigure 4 d) and
pull (P1 inFigure 4 b,d) aqueous flows, and a push oil flow (P0 in Figure 4 d). Since the top SiNx layer of the probe
is transparent for visible light, standard fluorescence microscopy
was used. Imaging and characterization of flows and droplets were
done with an inverted fluorescence microscope (IX73, Olympus) equipped
with a photomultiplier tube (PMT 2101, Thorlabs), video cameras (EOS
Rebel T7i, Canon, and Hero8, GoPro) and an LED lamp at 400 nm wavelength
(pE-300 White, CoolLED) for fluorescence excitation. To visualize
the flows (e.g.,Figure 4 d,e), a fluorescein solution in DI water was used at various concentrations
up to 1 mM as specified for each experiment (e.g.,Figure 4 d).
the fully packaged ND probe (
(Flow-EZ Module, LU-FEZ-7000, and LU-FEZ-N800, Fluigent) for push
(P2 in
pull (P1 in
is transparent for visible light, standard fluorescence microscopy
was used. Imaging and characterization of flows and droplets were
done with an inverted fluorescence microscope (IX73, Olympus) equipped
with a photomultiplier tube (PMT 2101, Thorlabs), video cameras (EOS
Rebel T7i, Canon, and Hero8, GoPro) and an LED lamp at 400 nm wavelength
(pE-300 White, CoolLED) for fluorescence excitation. To visualize
the flows (e.g.,
up to 1 mM as specified for each experiment (e.g.,
5
Planar pH Optical Sensor Imaging
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The planar pH optical sensors utilized the fluorophore HPTS (8-Hydroxypyrene-1,3,6-Trisulfonic Acid) and were modified after Zhu et al.1 (link). Fluorescence images were taken at 1–2 day intervals using a Canon camera (EOS Rebel T7i), after a ratiometric calibration with fluorescence emission ratio at 545 nm after 510 nm and 430 nm wavelength excitations1 (link). pH data reported here are on the NBS scale.
6
Longitudinal Assessment of Limb Regeneration
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At regular intervals over the 18-month maintenance period, animals were evaluated for soft tissue repatterning and bone regrowth. Animals were anesthetized as described previously, and high-resolution images of their wound sites were captured using a digital single-lens reflex (DSLR) camera (Canon EOS Rebel T7i) with a ruler in frame for calibrated measurements. To ensure replicability, the amputation plane served as a standard reference point for all measurements. The site of amputation was easily identified due a reliable tapering of the limb at the point of incision. Each measurement consisted of a linear assessment of length between the amputation site and the most distal end of the regenerate.
7
Optical Characterization of LCE Actuation
8
New Claviramus Species Identification
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Specimens were measured to record width of the middle of the thorax, trunk length (chaetiger 1 or collar to pygidium), radiolar crown length, number of radiolar pairs, number of thoracic and abdominal segments, and presence of gametes. The diagnosis and a full description of the new species were based on the holotype, with variation in the paratypes indicated in parentheses. The thoracic and abdominal glandular pattern was revealed by staining the worms with methyl green. Parts of thorax and abdomen of one paratype CBM-ZW 1124 were observed on the scanning electron microscope JSM-6500 at the Yokohama National University. Digital photographs were taken with an attached Canon EOS Rebel T7i digital camera. Type materials were deposited at the Natural History Museum and Institute, Chiba, Japan (catalogue code CBM-ZW) and at the Colección Poliquetológica, Universidad Autónoma de Nuevo León (catalogue code UANL). A key and a comparative table of diagnostic characters for species of Claviramus
9
Standardized Potato Imaging Protocol
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Potatoes from the greenhouse and the field were gently washed to remove clumps of dirt, allowed to dry and laid out a blue background along with a color card and an identification tag that was placed within a 3D printed tray positioned in the upper left corner of the image area. Images were acquired with a tripod mounted Canon EOS Rebel T7i. The camera setting was kept constant at F-stop 1/16, exposure time 0.8 s and ISO 100. Focal length was 50 cm. Each image was 6000 × 4000 pixels. A light box with fluorescent and LED lights positioned on the side and above the tubers was used to provide more even illumination and eliminate shadows.
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