Cck 8 assay kit

Cellular proliferation was evaluated using the CCK-8 assay kit (Transgen, China). Cells were plated in 96-well plates at a concentration of 3 × 10³ cells per well. Post-seeding, cells were incubated for specific durations:12, 24, 48, and 72 h. After each interval, CCK-8 reagent was added to the wells, and the plates were incubated at 37 °C for an additional 3 h. The optical density of each well was subsequently recorded at 450 nm using a microplate spectrophotometer.

A CCK-8 assay kit (TransGen Biotech # FC101-03, Beijing, China) was used to determine the effect of DCAF13 on cell proliferative abilities. Three siRNAs and siControl were transfected to knock down the expression of DCAF13 according to the manufacturer’s protocol. Cells were used for plate spreading 48 h after transfection. 2 × 10^³ transfected cells with 100 μl of culture medium were seeded and grown in 96-well plates, followed by incubation with CCK-8 solutions for 1 h at indicated periods (0, 2, 4, and 6 days). Subsequently, the absorbance value (450 nm) was measured using a microplate reader.

Cell proliferation was determined using the CCK 8 assay kit (Transgen, China). 96-well plates were seeded with 3 × 10³ cells per well. The cells were cultured for 24, 48, 72 and 96 h before being treated with CCK8 at 37 °C for 3 h. The absorbance at 450 nm was measured using a microplate reader.