Pizotifen

Serotonin hydrochloride, pizotifen and ADP were obtained from Sigma Aldrich (St. Louis, MO), cyproheptadine and EMD 281014 were obtained from Tocris Bioscience (Bristol, UK), clopidogrel was purchased from LKT Laboratories, Inc. (St. Paul, MN), stir bars and other disposables were from Chrono-Log (Havertown, PA), and U46619 was obtained from Cayman Chemical (Ann Arbor, MI). Src antibody, FITC-conjugated Annexin V, anti–P-selectin, and PAC-1 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). The anti-phosphotyrosine antibody was from BD Biosciences, (Franklin Lakes, NJ). Fura-2 acetoxymethyl ester (fura-2/AM) and Pluronic® F-127 were from Invitrogen (Grand Island, NY). The C57BL/6 mice were obtained from Jackson laboratory (Bar Harbor, ME). Platelet count was determined using an automated hematology analyzer (Drew Scientific Dallas, TX).

Amitriptyline, cyclobenzaprine hydrochloride, cyproheptadine, desloratadine, ketotifen, loratadine, nortriptyline, and pizotifen were purchased from Sigma Chemical (St. Louis, MO, USA). Azatadine was obtained from The United States Pharmacopeial Convention (USP; Rockville, MD, USA) and deptropine citrate from the European Directorate for the Quality of Medicines (EDQM; Strasbourg, France). Amineptine and rupatadine fumarate were purchased from Toronto Research Chemicals (North York, ON, Canada), and sorafenib and erlotinib were purchased from Cayman Chemical (Ann Arbor, MI, USA).