Sodium cyanide and cytochrome c from horse hearts

Reagents used in this study were purchased from the following sources: phenacetin (Nacalai Tesque, Kyoto, Japan); 4-acetamidophenol (Sigma-Aldrich, St. Louis, MO, USA); 4-acetamidophenol-d4 (Cayman Chemical, Ann Arbor, MI, USA); oxidized β-nicotinamide-adenine dinucleotide phosphate (NADP⁺), glucose-6-phosphate (G-6-P), glucose-6-phosphate dehydrogenase (G-6-PDH), reduced β-nicotinamide-adenine dinucleotide (NADH), oxidized β-nicotinamide-adenine dinucleotide, and reduced β-nicotinamide-adenine dinucleotide phosphate (NADPH) (Oriental Yeast, Tokyo, Japan); polyclonal anti-human CYP1A2 antibody (cat. no. ab170204; Abcam, Cambridge, UK); horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (ProteinSimple, Tokyo, Japan); and sodium cyanide and cytochrome c from horse hearts (Nacalai Tesque, Kyoto, Japan). All other chemicals and reagents were of the highest quality and commercially available.

Reagents were purchased from the following sources: (S)-warfarin, 7-ethoxycoumarin, and dimanganese decacarbonyl (Sigma-Aldrich, St. Louis, MO, USA); (S)-7-hydroxywarfarin (BD Gentest, Woburn, MA, USA); tolbutamide (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan); 4-hydroxytolbutamide and 4-hydroxytolbutamide-d9 (Toronto Research Chemicals, North York, ON, Canada); oxidized β-nicotinamide-adenine dinucleotide reduced form and β-nicotinamide-adenine dinucleotide phosphate reduced form (NADPH; Oriental Yeast, Tokyo, Japan); polyclonal anti-human CYP2C9 antibody (cat. no. ab4236; Abcam, Cambridge, UK); horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (ProteinSimple, Tokyo, Japan); and sodium cyanide and cytochrome c from horse hearts (Nacalai Tesque, Kyoto, Japan). All other chemicals and reagents were of the highest commercially available quality.