N alkanes c8 c20

The fresh S. rugoso-annulata was provided by Xinxin Vegetable Planting Cooperative (Meihe, Jilin, China). 2-Methyl-3-heptanone was purchased from Maclean Biochemical Technology Co., Ltd. (Shanghai, China). The n-alkanes (C₈~C₂₀) were purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). The 5′-nucleotides standards obtained from Sigma-Aldrich (St. Louis, MO, USA). The amino acids mixture standard solution was purchased from Waters (Shanghai, China). Flavourzyme^® was purchased from Solaibao Technology Co. (Beijing, China).

Nanfeng mandarins, Xunwu mandarins and Yangshuo kumquats were purchased from local fruit market in Ganzhou city in Jiangxi Province in November 2019. A physiological drop of Gannan Newhall navel oranges was collected from the orchard of Gannan Normal University in June 2019.
2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and n-alkanes (C₈–C₂₀) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Butylatedhydroxytoluene (BHT) was purchased from Macklin, Shanghai, China. Ampicillin (sodium salt) was purchased from Solarbio, Beijing, China. The following microorganisms were purchased from Beijing, China General Microbiological Culture Collection Center (CGMCC): Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923), Bacillus subtilis (ATCC6633), Salmonella typhimurium (ATCC14028) and Pseudomonas aeruginosa (ATCC9207).

The volatile components analysis of the saffron was carried out using gas chromatography-mass spectrometry (GC-MS) equipped with an Agilent 7890A system (A.01.01, Wilmington, DE, USA) and a mass selective detector 5975 Network MSD and coupled to a MPS automatic sampling system, as described previously by Naim et al. [62 (link)]. The chromatographic separation was performed on a HP-5MS capillary column (30 m × 0.25 mm, film thickness 0.17 mm), and the following temperature program was used: 60 °C held for 3 min, then increased to 210 °C at a rate of 4 °C/min, then held at 210 °C for 15 min, then increased to 300 °C at a rate of 10 °C/min, and finally held at 300 °C for 15 min. Helium was used as the carrier gas at a constant flow of 1 mL/min. For the quantification, the results are presented as a percentage of the peak area, considering a response factor of the fiber. Mass Hunter Version B.06.00 (Agilent Technologies) was used for the data acquisition and processing. The identification of the components was based on the comparison of the obtained mass spectrum with those from the commercial databases (NIST17 and Wiley) and by comparison with the retention index (RI) of each peak from the literature (Pherobase). The experimental retention index (RI) of the compounds were calculated following the injection of a mixture of n-alkanes C8-C20 (Sigma Aldrich, Darmstadt, Germany).