Live imaging was carried out by dechorionating embryos (stage 15), mounting them under Halocarbon oil, injecting 2–3% egg volume of LysoTracker (Molecular Probes) as described in Ref. (33 (link)). Recording started 30 min following injection.
Cy3 and cy5
Cy3 and Cy5 are fluorescent dyes commonly used in molecular biology and immunoassay techniques. Cy3 emits light in the orange-red region of the visible spectrum, while Cy5 emits light in the far-red region. These dyes are often used for labeling proteins, nucleic acids, and other biomolecules to facilitate detection and visualization in various applications.
Lab products found in correlation
3 protocols using cy3 and cy5
Immunohistochemistry Embryo Staining
Live imaging was carried out by dechorionating embryos (stage 15), mounting them under Halocarbon oil, injecting 2–3% egg volume of LysoTracker (Molecular Probes) as described in Ref. (33 (link)). Recording started 30 min following injection.
Immunolabeling of Neuronal Structures
Immunofluorescence Staining Protocol for α-Synuclein
antibodies. The free-floating sections were washed thrice with PBS from anti-freeze solution.
The sections were then incubated in 0.025% Triton X-100 (Sigma Aldrich) in PBS containing 5% of normal serums (Vector Laboratories Inc, US) matching the species used to raise the secondary antibody for that proper stainings for 1 h. Primary antibodies were diluted in PBS and overnight incubated at 4 °C. The next day, the sections were washed thrice with PBS for 5 min, then incubated with Cy3 and Cy5 (working dilution 1:300) fluorophore conjugated secondary antibodies (Jackson Immunoresearch, Sweden). The sections were mounted on positive charged superfrost plus glass slides (ThermoScientific, US) and cover-slipped using PVA/DABCO. The immunofluorescence stainings were visualized on a Leica SP8 laserscanning confocal microscope (Leica, Germany).
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