Anti cd10 pe

For cell sorting, cell suspension were incubated at 4°C with Fc-blocking reagent (Miltenyi Biotec) and stained for 30min with the following monoclonal antibodies: anti-CD45 AF700 (clone: HI30), anti-CD19 PE-Cy7 (clone: HIB19), anti-CD38APC-Cy7 (clone: HIT2), anti-CD10 PE (clone: HI10a), anti-IgM BV605 (clone: MHM-88) (all from Biolegend), anti-CD27 PerCpCy5.5 (clone: M-T271) (BD Biosciences), and anti-IgD FITC (Southern). CD45⁺CD19⁺CD38^dullCD10⁻IgD²⁺ IgM⁺CD27⁻ naive B cells, CD45⁺CD19⁺CD38^dullCD10⁻IgD⁺IgM²⁺CD27⁺ MZ B cells, CD45⁺CD19⁺CD38^intCD10⁺IgD-IgM⁺CD27⁺ GC-M, CD45⁺CD19⁺CD38^dullCD10⁻IgD⁻IgM⁺ CD27⁺ ME-M B cells, CD45⁺CD19⁺ CD38^dullCD10⁻ IgD⁻IgM⁻CD27⁺ ME-SW B cells, CD45⁺CD19⁺CD38^intCD10⁺IgD⁻CD27⁺ GC B cells, CD45⁺CD19⁺CD38²⁺CD10⁻IgD⁻IgM⁺CD27⁺ PC-M and CD45⁺CD19⁺CD38²⁺CD10⁻ IgD⁻IgM⁺CD27⁺ switched PC were sorted with a FACSAria II (BD Biosciences) after exclusion of dead cells through DAPI staining. For sorting of FCRL4⁺ and FCRL4^- ME-M, anti-FCRL4 APC (clone: 413D12) was added to the ‘cocktails’. The purity of cells sorted this way was consistently > 95%.