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F4 80 clone ci a3 1

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F4/80 (clone CI: A3-1) is a monoclonal antibody that recognizes the F4/80 antigen, a glycoprotein expressed on the surface of mature mouse macrophages. It is commonly used as a marker for the identification and characterization of mouse macrophages in various research applications.

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Lab products found in correlation

Mhc 2 clone m5 114, by Thermo Fisher Scientific (2 mentions) Gr 1 clone rb6 8c5, by BioLegend (2 mentions) Cd45 clone 30 f11, by Thermo Fisher Scientific (2 mentions) Cd11b clone m1 70, by Thermo Fisher Scientific (2 mentions) Cd115 clone afs98, by Thermo Fisher Scientific (2 mentions) Cd3e clone 145 2c11, by BioLegend (2 mentions) Lsrii fortessa, by BD (2 mentions)

2 protocols using f4 80 clone ci a3 1

1

Isolation and Characterization of Microglia

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Mice were deeply anesthetized with a lethal dose of choral hydrate and transcardially perfused with 0.1 M PBS. Brain was removed and one forebrain hemisphere (without olfactory bulb) was minced, incubated in phenol-red free DMEM supplemented with 2% heat inactivated FBS, 10mM HEPES and Collagenase type IV (0.4 mg/mL) for 15 min and then passed through a 19G blunt syringe to obtain a homogeneous cell suspension. Mononuclear cells were separated with a 40% Percoll gradient. Isolated cells were surface stained in FACS buffer for 20–30 min on ice with the following antibodies: CD11b (clone M1/70, eBioscience), F4/80 (clone CI: A3-1, BioRad), CD45 (clone 30F11, eBioscience), MHC II (clone M5/114.15.2, eBioscience), CD3e (clone 145-2C11, Biolegend), Gr-1 (clone RB6–8C5, Biolegend), CD115 (clone AFS98, eBioscience). Multiparameter analysis was performed on a LSR II Fortessa (BD) and analyzed with FlowJo software (Tree Star) (Details are included in the Flow Cytometry Reporting Summary). Dead cells and doublets were excluded from all analysis.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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2

Isolation and Characterization of Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were deeply anesthetized with a lethal dose of choral hydrate and transcardially perfused with 0.1 M PBS. Brain was removed and one forebrain hemisphere (without olfactory bulb) was minced, incubated in phenol-red free DMEM supplemented with 2% heat inactivated FBS, 10mM HEPES and Collagenase type IV (0.4 mg/mL) for 15 min and then passed through a 19G blunt syringe to obtain a homogeneous cell suspension. Mononuclear cells were separated with a 40% Percoll gradient. Isolated cells were surface stained in FACS buffer for 20–30 min on ice with the following antibodies: CD11b (clone M1/70, eBioscience), F4/80 (clone CI: A3-1, BioRad), CD45 (clone 30F11, eBioscience), MHC II (clone M5/114.15.2, eBioscience), CD3e (clone 145-2C11, Biolegend), Gr-1 (clone RB6–8C5, Biolegend), CD115 (clone AFS98, eBioscience). Multiparameter analysis was performed on a LSR II Fortessa (BD) and analyzed with FlowJo software (Tree Star) (Details are included in the Flow Cytometry Reporting Summary). Dead cells and doublets were excluded from all analysis.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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