I.p. injections of tamoxifen from MP Biomedicals at 5 µl per gram body weight of 20 mg/ml diluted in corn oil were administrated to 2-mo-old mice daily for 4 d before injury. For muscle injury experiments, mice were anaesthetized by i.p. injection of Ketamin-Xylazin (Centravet) at 10 µl per gram body weight of 80–10 mg/ml diluted in saline. Mouse legs were cleaned with alcohol and tibialis anterior muscles were injected with 35 µl of cardiotoxin solution (12 µM, diluted in saline; Latoxan) using an insulin needle (3/10 cc insulin syringe; BD). For in vivo proliferation assays, we used four i.p. injections of BrdU at 5 µl per gram of body weight of 10 mg/ml diluted in saline.
Cc insulin syringe
Manufactured by BD
Sourced in United States
The 3/10 cc insulin syringe is a medical device designed for the accurate and precise delivery of small volumes of insulin or other medications. It has a capacity of 3/10 cubic centimeters (cc) or 0.3 milliliters (mL). The syringe features a graduated scale to ensure dosage accuracy.
Automatically generated - may contain errors
2 protocols using cc insulin syringe
1
Muscle Injury and Regeneration Protocols
2
In vivo Bioluminescent Imaging of mRNA-LNPs
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All animal procedures were overseen by the Institutional Animal Care and Use Committee (IACUC) of the Singapore Biological Resource Centre (BRC, approved protocol number: 221681). Female BALB/cAnNTac (BALB/c) mice at 5–6 weeks of age were obtained from InVivos Pte Ltd (Singapore) and maintained in a specific pathogen-free facility of BRC. Ten BALB/c mice were inoculated with FLuc mRNA-LNPs containing 1 μg of mRNA in 20 μL via subcutaneous injection in the hind leg area using a 3/10cc insulin syringe (BD, USA). Five naive mice injected with luciferin served as a mock control. At indicated time points, VivoGlo luciferin (Promega, USA) was intraperitoneally injected into the mice at a dose of 150 mg per kg. After 10 min, the IVIS Spectrum imaging system (PerkinElmer, USA) was employed to obtain the luminescence signals of the mice. Organs (spleen, liver, kidney, lung, heart, inguinal lymph node, and skin) were harvested immediately for ex vivo luminescence imaging. The total flux values in each region of interest were measured using the Living Image software (PerkinElmer, USA).
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