Annexin 5 fitc propidium iodide

Manufactured by Dojindo Laboratories

Sourced in Japan

Annexin V-FITC/propidium iodide (PI) is a fluorescent staining kit used for the detection of apoptosis and cell viability. Annexin V binds to phosphatidylserine, a phospholipid that is exposed on the surface of apoptotic cells. FITC-conjugated Annexin V is used to label these cells. Propidium iodide (PI) is a DNA-binding dye that stains necrotic or late apoptotic cells. The kit allows for the simultaneous detection of early apoptotic, late apoptotic, and necrotic cells through flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Cell counting kit 8 cck 8 kit, by Dojindo Laboratories (1 mentions) Cgamp, by Biolog (1 mentions) Dcfh da, by Merck Group (1 mentions) Calcein am propidium iodide, by Keygen Biotech (1 mentions) Sodium borohydride, by Sinopharm (1 mentions) Triethanolamine, by Sinopharm (1 mentions) Hexadecyl trimethyl ammonium chloride, by Sinopharm (1 mentions)

2 protocols using annexin 5 fitc propidium iodide

Evaluating Calycosin-Induced Apoptosis

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In all, 1.5–2×10⁵ cells were plated into six-well plates, incubated with calycosin (0–800 μM) for 24 hours, and then collected and stained with Annexin V-FITC/propidium iodide (PI) as per the manufacturer’s instructions (Dojindo, Kumamoto, Japan). Briefly, the cells were washed twice in cold phosphate-buffered saline (PBS), resuspended in 100 μL binding buffer, and incubated with 5 μL Annexin V and 5 μL PI for 15 minutes at room temperature in the dark before flow cytometric analysis.

Nie X.H., Ou-yang J., Xing Y., Li D.Y., Liu R.E, & Xu R.X. (2016). Calycosin inhibits migration and invasion through modulation of transforming growth factor beta-mediated mesenchymal properties in U87 and U251 cells. Drug Design, Development and Therapy, 10, 767-779.

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Multimodal Nanomedicine Evaluation

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Tetraethylorthosilicate (TEOS), 3‐aminopropyltrimethoxysilane (APTES), DAPI, and 2, 7‐dichlorodihydrofluorescein diacetate (DCFH‐DA) were purchased from Sigma‐Aldrich. Hexadecyl trimethylammonium chloride (CTAC), triethanolamine (TEA), sodium borohydride (NaBH₄), and chloroplatinic acid (H₂PtCl₆) were purchased from Sinopharm Chemical Reagent Co. Ltd. CalceinAM/ propidium iodide (PI) was purchased from KeyGen BioTech (Shanghai, China). Annexin V‐FITC/propidium iodide (PI) and Cell Counting Kit 8 (CCK8) kit were obtained from Dojindo Laboratories. αPD1 was purchased from BioLegend, Inc (USA). cGAMP and its fluorescent analogue were purchased from BIOLOG Life Science Institute (Germany).

Wang L., Zhou H., Chen Q., Lin Z., Jiang C., Chen X., Chen M., Liu L., Shao L., Liu X., Pan J., Wu J., Song J., Wu J, & Zhang D. (2023). STING Agonist‐Loaded Nanoparticles Promotes Positive Regulation of Type I Interferon‐Dependent Radioimmunotherapy in Rectal Cancer. Advanced Science, 11(7), 2307858.

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