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Apoptag peroxidase in situ apoptosis detection kit

Manufactured by Promega
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The ApopTag Peroxidase in situ Apoptosis Detection Kit is a laboratory tool used to identify and quantify apoptosis, a form of programmed cell death, in tissue samples. The kit provides the necessary reagents to detect and visualize apoptotic cells through enzymatic staining methods.

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Apoptosis detection kit (12 citations)

2 protocols using apoptag peroxidase in situ apoptosis detection kit

1

Apoptosis Evaluation in Tumor Cells

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Occurrence of apoptosis was investigated by TUNEL assay on cells treated with 9, 10, 10 μM GW627368X for HeLa, SiHa and ME 180 respectively for 0, 24 and 48 h and on tissue sections of excised tumors using ApopTag Peroxidase in situ Apoptosis Detection Kit (Promega) following kit protocol.23 Cells were analyzed by confocal laser scanning microscope at × 20 magnification.
Parida S., Pal I., Parekh A., Thakur B., Bharti R., Das S, & Mandal M. (2016). GW627368X inhibits proliferation and induces apoptosis in cervical cancer by interfering with EP4/EGFR interactive signaling. Cell Death & Disease, 7(3), e2154-.
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2

Detecting Apoptosis with TUNEL Assay

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Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assays were performed using the ApopTag Peroxidase in situ Apoptosis Detection Kit (Promega, Madison, WI, USA) according to the instructions of the manufacturer. Propidium iodide was used as a counterstain. Cells were analysed by confocal laser scanning microscopy at 20 × magnification.
Pal I., Sarkar S., Rajput S., Dey K.K., Chakraborty S., Dash R., Das S.K., Sarkar D., Barile E., De S.K., Pellecchia M., Fisher P.B, & Mandal M. (2014). BI-69A11 enhances susceptibility of colon cancer cells to mda-7/IL-24-induced growth inhibition by targeting Akt. British Journal of Cancer, 111(1), 101-111.
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