3 iodo l tyrosine

Manufactured by Merck Group

Sourced in United States

3-iodo-L-tyrosine is a laboratory reagent used in biochemical and cell biology research. It is a halogenated amino acid derivative. No further details on its intended use or applications can be provided without extrapolation.

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L-tyrosine (227 citations) Tyrosine (137 citations) 3-iodo-L-tyrosine (3IY) (3 citations)

6 protocols using 3 iodo l tyrosine

Neurotransmitter Signaling Assay Protocol

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Guanethidine, phentolamine, phenylephrine, noradrenaline, N-Methyl-D-glucamine, tetrodotoxin and 3-iodo-L-Tyrosine were purchased from Sigma-Aldrich Chemicals Co. (Missouri, USA). A-803467 and salsolinol were bought from Tocris Bioscience (Bristol, UK). Chicken anti-tyrosine hydroxylase and goat polyclonal secondary antibody to chicken IgY—H&L (Alexa Fluor® 594) were acquired from Novus Biologicals (Colorado-USA) and Abcam (Massachusetts, USA), respectively.

Campos R., Mónica F.Z., Rodrigues R.L., Rojas-Moscoso J.A., Moreno R.A., Cogo J.C., de Oliveira M.A., Antunes E, & De Nucci G. (2017). Tetrodotoxin-insensitive electrical field stimulation-induced contractions on Crotalus durissus terrificus corpus cavernosum. PLoS ONE, 12(8), e0183766.

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Tyrosine-Supplemented Defined Media Preparation

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MOPS-EZ Rich Defined Media (RDM, TEKnova) with the full complement of amino acids (RDM-20), as well as the knockout medias (RDM-19 and RDM-13), were prepared according the manufacturers specification. For media preparation, 3-nitro-L-tyrosine or 3-iodo-L-tyrosine (Sigma-Aldrich) was added to ultrapure deionized water to a final concentration of 17.24 mM. The 3nY or 3iY supplemented water was used to complete RDM, and the entire preparation was filter sterilized with Nalgene Rapid-Flow SFCA filtration units. Prepared media was stored at 4 °C, and moved to room temperature 16–24 hours before use.

Tack D.S., Cole A.C., Shroff R., Morrow B.R, & Ellington A.D. (2018). Evolving Bacterial Fitness with an Expanded Genetic Code. Scientific Reports, 8, 3288.

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Inhibition of TH Activity in B Cells

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TH activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalog number: 18250), 30 min before activation with anti-IgM/CpG.

Honke N., Lowin T., Opgenoorth B., Shaabani N., Lautwein A., Teijaro J.R., Schneider M, & Pongratz G. (2022). Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biology, 20(1), e3001513.

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Tyrosine Hydroxylase Inhibition in Drosophila

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For the pharmacological manipulations 1% agar with 5% sucrose was used as a vehicle medium. The tyrosine hydroxylase inhibitor, 3-iodo-L-tyrosine (Sigma-Aldrich, Stockholm, Sweden) was mixed into the medium to a final concentration of 3 mM. There day old male flies were transferred and kept on the media for 48 hours.

Williams M.J., Klockars A., Eriksson A., Voisin S., Dnyansagar R., Wiemerslage L., Kasagiannis A., Akram M., Kheder S., Ambrosi V., Hallqvist E., Fredriksson R, & Schiöth H.B. (2016). The Drosophila ETV5 Homologue Ets96B: Molecular Link between Obesity and Bipolar Disorder. PLoS Genetics, 12(6), e1006104.

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Enzymatic Tyrosine Halogenation Kinetics

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The enzyme mushroom tyrosinase (EC 1.14.18.1, 1715 U/mg) and 3′-iodo-l-tyrosine were from Sigma. Deuterated water (99.9% D) and Amberlite IR-120 (Na⁺) resin were from Aldrich. Silica gel plates (silica gel 60 F₂₅₄) were purchased from Merck. Deuterated 30% KO²H/²H₂O and 85% ²H₃PO₄/²H₂O were obtained from POLATOM, Poland. 3′-Fluoro-l-tyrosine and 3′-chloro-l-tyrosine were purchased from Alfa Aesar.

Pająk M, & Kańska M. (2017). Isotope effects in mechanistic studies of l-tyrosine halogen derivatives hydroxylation catalyzed by tyrosinase. Journal of Radioanalytical and Nuclear Chemistry, 314(3), 2123-2128.

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Synthesis of Halogenated Tyrosine Derivatives

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The solution of 37% 2 HCl/ 2 H 2 O was purchased from POLATOM (Poland). 2 H 2 O (99.9% 2 H) and Amberlite IR-120 (Na + ) resin were purchased from Aldrich. Plastic TLC plates, silica gel 60, coated with fluorescent indicator F 254 were from Merck. 3′-Fluoro-L-tyrosine and 3′-chloro-L-tyrosine were purchased from Alfa Aesar and 3′-iodo-Ltyrosine was from Sigma.

Pająk M. (2020). Microwave enhanced synthesis of halogenated derivatives of L-tyrosine labeled with deuterium in aromatic ring. Journal of Radioanalytical and Nuclear Chemistry, 326(1), 857-860.

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