Mca579

Manufactured by BD

The MCA579 is a laboratory instrument designed for automated cell analysis and counting. It utilizes advanced imaging and software technologies to provide accurate and reliable data on cell populations. The core function of the MCA579 is to quantify and characterize various cell types within a sample.

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Lab products found in correlation

Automacs system, by Miltenyi Biotec (1 mentions) Anti mouse igg1 microbeads, by Miltenyi Biotec (1 mentions) Anti cd77, by Bio-Rad (1 mentions) Anti fitc microbeads, by Miltenyi Biotec (1 mentions) Anti igd fitc, by BD (1 mentions)

2 protocols using mca579

Isolation of Human and Murine B Cell Populations

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Human B cell populations were affinity-purified from de-identified human tonsillectomy specimens using standard protocols^{28 (link)} with approval from the Human Research Protections Programs, Division of Research Integrity of the Weill Cornell Medical College, in accordance with the Declaration of Helsinki. naive B and GC B cell purity ( > 90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579) and CD38 (BD Biosciences 340439).
Murine B cell populations from immunized mice and organoid and 2D cultures were purified by FACS using FAS⁻ GL7⁻ IgD⁺ B220⁺ splenocytes as naive B cells, and FAS⁺ GL7⁺ B220⁺ splenocytes as GC B cells.

Béguelin W., Rivas M.A., Calvo Fernández M.T., Teater M., Purwada A., Redmond D., Shen H., Challman M.F., Elemento O., Singh A, & Melnick A.M. (2017). EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop. Nature Communications, 8, 877.

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Isolation of Naive and Germinal Center B Cells

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Tonsils were minced, and mononuclear cells were isolated using Ficoll Histopaque density centrifugation. Naïve B cells were separated by positive selection using AutoMACS system (Miltenyi Biotec) after incubation with anti-IgD-FITC (BD Pharmingen) followed by anti-FITC microbeads (Miltenyi Biotec). Germinal center B cells (GCB) were separated by positive selection with anti-CD77 (AbD Serotec) followed by mouse anti-IgM, IgG1 isotype (BD Pharmingen) and anti-mouse-IgG1 microbeads (Miltenyi Biotec). Naive and germinal center B cell purity (> 90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579) and CD38 (BD Biosciences 340439). GCB were further verified by expression of A4GALT and BCL6 by RT-qPCR.

Calvo-Vidal M.N., Zamponi N., Krumsiek J., Stockslager M.A., Revuelta M.V., Phillip J.M., Marullo R., Tikhonova E., Kotlov N., Patel J., Yang S.N., Yang L., Taldone T., Thieblemont C., Leonard J.P., Martin P., Inghirami G., Chiosis G., Manalis S.R, & Cerchietti L. (2021). Oncogenic HSP90 Facilitates Metabolic Alterations in Aggressive B-cell Lymphomas. Cancer Research, 81(20), 5202-5216.

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