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Superdex 200i 10x300gl column

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The Superdex 200i 10X300GL column is a size-exclusion chromatography column designed for the separation and purification of proteins, peptides, and other biomolecules. The column has a bed volume of 24 ml and a bed height of 300 mm. It is compatible with FPLC (Fast Protein Liquid Chromatography) systems and can be used for a wide range of applications.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (2 mentions) Quikchange site directed mutagenesis kit, by Agilent Technologies (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) α mem, by Thermo Fisher Scientific (2 mentions) Collagenase type 2, by Worthington (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions) Glutamine, by Thermo Fisher Scientific (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Ap20187, by Takara Bio (2 mentions) Celltracker cm dii dye, by Thermo Fisher Scientific (2 mentions) Diaphorase, by Merck Group (2 mentions) Recombinant mouse fcγr 1 and fcγr 4, by ACROBiosystems (2 mentions) Mabselect sure resin, by Merck Group (2 mentions) Recombinant human and mouse cd38 proteins, by R&D Systems (2 mentions) Nmnat1, by R&D Systems (2 mentions) Anti mouse tnfα and il6 neutralizing antibodies, by R&D Systems (2 mentions) Luminex premixed multi analyte magnetic luminex assay kit, by R&D Systems (2 mentions) Olaparib, by LC Laboratories (2 mentions)

4 protocols using superdex 200i 10x300gl column

1

Detailed Multidisciplinary Research Protocol

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The following reagents were used: AP20187 (Clontech), 78c (Calbiochem), Olaparib (LC Laboratories), type II collagenase (Worthington Biochemical), QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies), CellTracker CM-DiI dye (Invitrogen), recombinant mouse FcγR I and FcγR IV (Acro Biosystems), Isatuximab (TeneoBio), UniAb clone ID 337468 and 337469 (TeneoBio), MabSelect SuRe resin, Superdex 200i 10X300GL column, ADH, Diaphorase, 3TC, and HISTOPAQUE-1077 were from Sigma-Aldrich. Recombinant human and mouse CD38 proteins, NMNAT1, anti-mouse TNFα- and IL6-neutralizing antibodies, and Luminex Premixed Multi-Analyte Magnetic Luminex Assay Kit were from R&D Systems. αMEM, DMEM, DMEM:F12, fetal calf serum, glutamine, ITS, penicillin/streptomycin, and Lipofectamine 3000 were from Life Technologies. When not specified, reagents and chemicals were purchased from Sigma-Aldrich.
Chini C.C., Peclat T.R., Warner G.M., Kashyap S., Espindola-Netto J.M., de Oliveira G.C., Gomez L.S., Hogan K.A., Tarragó M.G., Puranik A.S., Agorrody G., Thompson K.L., Dang K., Clarke S., Childs B.G., Kanamori K.S., Witte M.A., Vidal P., Kirkland A.L., De Cecco M., Chellappa K., McReynolds M.R., Jankowski C., Tchkonia T., Kirkland J.L., Sedivy J.M., van Deursen J.M., Baker D.J., van Schooten W., Rabinowitz J.D., Baur J.A, & Chini E.N. (2020). CD38 ecto-enzyme in immune cells is induced during aging regulating NAD+ and NMN levels. Nature metabolism, 2(11), 1284-1304.
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2

Detailed Multidisciplinary Research Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were used: AP20187 (Clontech), 78c (Calbiochem), Olaparib (LC Laboratories), type II collagenase (Worthington Biochemical), QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies), CellTracker CM-DiI dye (Invitrogen), recombinant mouse FcγR I and FcγR IV (Acro Biosystems), Isatuximab (TeneoBio), UniAb clone ID 337468 and 337469 (TeneoBio), MabSelect SuRe resin, Superdex 200i 10X300GL column, ADH, Diaphorase, 3TC, and HISTOPAQUE-1077 were from Sigma-Aldrich. Recombinant human and mouse CD38 proteins, NMNAT1, anti-mouse TNFα- and IL6-neutralizing antibodies, and Luminex Premixed Multi-Analyte Magnetic Luminex Assay Kit were from R&D Systems. αMEM, DMEM, DMEM:F12, fetal calf serum, glutamine, ITS, penicillin/streptomycin, and Lipofectamine 3000 were from Life Technologies. When not specified, reagents and chemicals were purchased from Sigma-Aldrich.
Chini C.C., Peclat T.R., Warner G.M., Kashyap S., Espindola-Netto J.M., de Oliveira G.C., Gomez L.S., Hogan K.A., Tarragó M.G., Puranik A.S., Agorrody G., Thompson K.L., Dang K., Clarke S., Childs B.G., Kanamori K.S., Witte M.A., Vidal P., Kirkland A.L., De Cecco M., Chellappa K., McReynolds M.R., Jankowski C., Tchkonia T., Kirkland J.L., Sedivy J.M., van Deursen J.M., Baker D.J., van Schooten W., Rabinowitz J.D., Baur J.A, & Chini E.N. (2020). CD38 ecto-enzyme in immune cells is induced during aging regulating NAD+ and NMN levels. Nature metabolism, 2(11), 1284-1304.
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3

Efficient UniAb Purification from CHO Cells

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Soluble UniAb was expressed in CHO cells using protein free media supplemented with L-Glutamine (Invitrogen). Cell harvest was clarified by centrifugation and 0.2 μm PES filtration. During purification, the first capture step employed a MabSelect SuRe (Sigma-Aldrich) resin. The column was equilibrated with PBS and the clarified harvest loaded onto the column. A post load wash with PBS occurred, followed by elution with 50 mM Acetic Acid, 10% Glycerol, 10% Sucrose, pH 3.6. The elution pool was immediately neutralized to approximately pH 6.5 with 2 M Tris, pH 9. After the first capture step, the protein pool was concentrated and loaded on to a Superdex 200i 10X300GL column (Sigma-Aldrich) equilibrated with PBS for further polishing to remove any high molecular weight species. Fractions were collected, and the most homogenous fractions were pooled.
Chini C.C., Peclat T.R., Warner G.M., Kashyap S., Espindola-Netto J.M., de Oliveira G.C., Gomez L.S., Hogan K.A., Tarragó M.G., Puranik A.S., Agorrody G., Thompson K.L., Dang K., Clarke S., Childs B.G., Kanamori K.S., Witte M.A., Vidal P., Kirkland A.L., De Cecco M., Chellappa K., McReynolds M.R., Jankowski C., Tchkonia T., Kirkland J.L., Sedivy J.M., van Deursen J.M., Baker D.J., van Schooten W., Rabinowitz J.D., Baur J.A, & Chini E.N. (2020). CD38 ecto-enzyme in immune cells is induced during aging regulating NAD+ and NMN levels. Nature metabolism, 2(11), 1284-1304.
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4

Efficient UniAb Purification from CHO Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Soluble UniAb was expressed in CHO cells using protein free media supplemented with L-Glutamine (Invitrogen). Cell harvest was clarified by centrifugation and 0.2 μm PES filtration. During purification, the first capture step employed a MabSelect SuRe (Sigma-Aldrich) resin. The column was equilibrated with PBS and the clarified harvest loaded onto the column. A post load wash with PBS occurred, followed by elution with 50 mM Acetic Acid, 10% Glycerol, 10% Sucrose, pH 3.6. The elution pool was immediately neutralized to approximately pH 6.5 with 2 M Tris, pH 9. After the first capture step, the protein pool was concentrated and loaded on to a Superdex 200i 10X300GL column (Sigma-Aldrich) equilibrated with PBS for further polishing to remove any high molecular weight species. Fractions were collected, and the most homogenous fractions were pooled.
Chini C.C., Peclat T.R., Warner G.M., Kashyap S., Espindola-Netto J.M., de Oliveira G.C., Gomez L.S., Hogan K.A., Tarragó M.G., Puranik A.S., Agorrody G., Thompson K.L., Dang K., Clarke S., Childs B.G., Kanamori K.S., Witte M.A., Vidal P., Kirkland A.L., De Cecco M., Chellappa K., McReynolds M.R., Jankowski C., Tchkonia T., Kirkland J.L., Sedivy J.M., van Deursen J.M., Baker D.J., van Schooten W., Rabinowitz J.D., Baur J.A, & Chini E.N. (2020). CD38 ecto-enzyme in immune cells is induced during aging regulating NAD+ and NMN levels. Nature metabolism, 2(11), 1284-1304.
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