Syn 1

Manufactured by Merck Group

SYN-1 is a laboratory equipment designed for the synthesis of chemical compounds. It provides a controlled environment for conducting chemical reactions. The core function of SYN-1 is to facilitate the process of synthesizing new compounds or modifying existing ones in a laboratory setting.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (3 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Psd 95, by Merck Group (1 mentions) Brain derived neurotrophic factor (bdnf), by Abcam (1 mentions) Gapdh, by Merck Group (1 mentions) Vglut2, by Merck Group (1 mentions) Alexa fluor conjugated antibody, by Thermo Fisher Scientific (1 mentions) Glur1, by Abcam (1 mentions) Homer 1, by R&D Systems (1 mentions) Psd95, by Cell Signaling Technology (1 mentions) Glun2b, by Proteintech (1 mentions) Glun2a, by Proteintech (1 mentions) Goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Phosstop cocktail, by Roche (1 mentions) Glun1, by Cell Signaling Technology (1 mentions) Glua3, by Merck Group (1 mentions) Mtor inhibitor rapamycin, by MedChemExpress (1 mentions) β actin, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Complete protease inhibitor, by Roche (1 mentions)

4 protocols using syn 1

Hippocampal and Cortical Protein Analysis

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Brain hippocampal and cortical tissues were homogenized and Western blotting performed as previously described (5 (link)) using rabbit polyclonal antibodies Synapsin-1 (SYN-1, 1:200; Millipore), BDNF (Abcam, 1:200), pCREB and CREB (Chemicon, 1000), and mouse monoclonal antibodies PSD95 (1:200; Millipore) and GAPDH (1:200; Millipore). Blots were scanned using a LiCor Odyssey Infrared Imaging System. Intensity of bands was measured by LiCor Odyssey software.

Shi Q., Chowdhury S., Ma R., Le K.X., Hong S., Caldarone B.J., Stevens B, & Lemere C.A. (2017). Complement C3 deficiency protects against neurodegeneration in aged plaque-rich APP/PS1 mice. Science translational medicine, 9(392), eaaf6295.

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Immunofluorescent Mapping of Synaptic Markers

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Immunofluorescent staining of pre- and post-synaptic markers and
analyses were performed as previous described [44 (link), 48 ].
Fixed, frozen brain sections were dried, washed in Tris-buffered saline
(TBS), and blocked with 20% goat serum + 0.3% Triton X-100 in TBS for 2 h.
Primary antibodies were diluted in antibody buffer with 0.03% Triton X-100
and 10% goat serum as follows: Vglut2 (Millipore goat anti-guinea pig,
1:1000), Homer1 (R&D Systems goat anti-mouse, 1:200), GluR1 (Abcam goat
anti-rabbit, 1:200), and SYN-1 (Millipore goat anti-rabbit, 1:200) and
incubated overnight at 4°C. Secondary Alexa Fluor-conjugated
antibodies (Life Technologies) were added at 1:200 in 0.03% Triton X-100
with 10% goat serum for 2 h at room temperature (RT). Confocal imaging was
performed using a ZEISS LSM710 confocal microscope and a 63x oil objective.
Images were acquired using a 1 Airy Unit (AU) pinhole, while holding the
gain and offset parameters constant for all sections and mice for each
experiment.

Sun T., Shi Q., Zhang Y., Power C., Hoesch C., Antonelli S., Schroeder M.K., Caldarone B.J., Taudte N., Schenk M., Hettmann T., Schilling S., McDannold N.J, & Lemere C.A. (2021). Focused ultrasound with anti-pGlu3 Aβ enhances efficacy via recruitment of peripheral immune cells. Journal of controlled release : official journal of the Controlled Release Society, 336, 443-456.

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Quantifying Synaptic Proteins in Tissues

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Antibodies against RAB39B (Cat# D-12162-1-AP), GluA2 (Cat# 11994-1-AP), GluN2A (Cat# 19953-1-AP), GluN2B (Cat# 19954-1-AP), and tyrosine hydroxylase (TH, Cat# 66334-1-IG) were from Proteintech. Antibodies against GFAP (Cat# 3670s), β-actin (Cat# 8457s), p62 (Cat# 5114s), GluN1 (Cat# 5704s), PSD95 (Cat# 3450s), LC3B (Cat# 3868s), S6 (Cat# 2217s), and phosphorylated S6 (Ser240/244) (Cat# 5364s) were from Cell Signaling Technology. Antibodies against GluA1 (Cat# 04-855), GluA3 (Cat# MAB5416), and SYN1 (Cat# AB1543) were from Millipore. Antibodies against NeuN (Cat# ab177487), Iba1 (Cat# 016-20001), and synaptophysin (SYP) (Cat# S5768) were from Abcam, Wako, and Sigma-Aldrich, respectively. Goat anti-rabbit (Cat# 31460) or anti-mouse (Cat# 31430) IgG (HCL) secondary antibodies conjugated with horseradish peroxidase were from Thermo Fisher Scientific.
The mTOR inhibitor rapamycin (Cat# HY-10219) was from MedChemExpress. DMSO (Cat# 20688) was from Thermo Fisher Scientific. 4′,6-diamidino-2-phenylindole (DAPI) (Cat# D9542) was from Sigma-Aldrich. Complete Protease Inhibitor and PhosSTOP Cocktails were from Roche.
Different protein levels in mouse tissues and cells were determined by western blot. Detailed procedures are presented in Supplementary Materials and Methods. Protein band intensity was quantified by densitometry using the Image J software (National Institutes of Health).

Niu M., Zheng N., Wang Z., Gao Y., Luo X., Chen Z., Fu X., Wang Y., Wang T., Liu M., Yao T., Yao P., Meng J., Zhou Y., Ge Y., Wang Z., Ma Q., Xu H, & Zhang Y.W. (2020). RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy. Frontiers in Cell and Developmental Biology, 8, 598622.

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Immunostaining Protocol for Cell Characterization

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Immunostaining was performed as previously described (Gao et al., 2017 (link)). Briefly, cells for immunostaining were seeded onto Matrigel pretreated coverslips. After aspiration of medium, cells were fixed with 4% PFA for 15 min at room temperature. Then cells were treated with permeabilization and blocking buffer (1% BSA and 0.5% Triton-X 100 in PBS) for 1 h at room temperature. Primary antibodies were incubated overnight at 4°C. The next day, fluorescent-dye conjugated secondary antibodies were incubated for 1 h at room temperature. Slides were mounted and images were captured with Leica SP8 microscope. The following antibodies were used in this study: TOM20 (Santa Cruz, #sc-17764), ATP5A1 (Abclonal, #5884), LAMP1 (Abcam, #24170), OCT4 (Santa Cruz, #sc-9081), NANOG (Santa Cruz, #sc-33760), SOX2 (Santa Cruz, #sc-17320), S100B (Sigma, #S2532), GFAP (Dako, #Z033401; Thermo Fisher, #13-0300), CRYAB (Santa Cruz, #sc-137143), TUJ1 (Biolegend, #801202), DCX (Santa Cruz, #sc-8066), MAP2 (Millipore, #AB5622), NEUN (Millipore, #ABN78), and SYN1 (Millipore, #Ab1543).

Gao L., Zhang Z., Lu J, & Pei G. (2019). Mitochondria Are Dynamically Transferring Between Human Neural Cells and Alexander Disease-Associated GFAP Mutations Impair the Astrocytic Transfer. Frontiers in Cellular Neuroscience, 13, 316.

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