Ac p53

Manufactured by Abcam

Sourced in United States

Ac-P53 is an antibody that specifically recognizes acetylated p53 protein. p53 is a tumor suppressor protein that is acetylated in response to various cellular stresses. The Ac-P53 antibody can be used to detect and quantify acetylated p53 in biological samples.

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Lab products found in correlation

Sirt1, by Abcam (3 mentions) Vcam 1, by Santa Cruz Biotechnology (2 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Chloroquine diphosphate, by Merck Group (1 mentions) P62 18420 1 ap, by Proteintech (1 mentions) Foxo1, by Abcam (1 mentions) P ampk, by Cell Signaling Technology (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) P ampk, by Abcam (1 mentions) Ac p53, by Cell Signaling Technology (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Icam 1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-Ac-p53

5 protocols using ac p53

Quantification of AMPK, p53, and SIRT1 Signaling

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Total AMPK, phosphorylation of AMPK (pAMPK), AcP53, FOXO1, and SIRT1 were assayed by Western blot analysis. Twenty micrograms (for AMPK, pAMPK, and FOXO1), 10 μg (for AcP53), and 40 μg (for SIRT1) of protein were loaded, and antibodies (AMPK, pAMPK, FOXO1, SIRT1 (Cell Signaling, Danvers, Mass. USA) and AcP53 (Abcam, Cambridge, Mass. USA)) were used in 1:1000 (AMPK, pAMPK, and SIRT1) and 1:2000 (AcP53, FOXO1) concentrations.

Toklu H.Z., Scarpace P.J., Sakarya Y., Kirichenko N., Matheny M., Bruce E.B., Carter C.S., Morgan D, & Tümer N. (2016). Intracerebroventricular tempol administration in older rats reduces oxidative stress in the hypothalamus but does not change STAT3 signalling or SIRT1/AMPK pathway. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 42(1), 59-67.

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Western Blot Analysis of Protein Levels

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Western blot analysis was performed using cell lysates, as described in our previous studies,5, 26, 31 with antibodies against ac‐P53 (1:500; Abcam), GAPDH (1:3000; Santa Cruz Biotechnology), P53 (1:1000; Cell Signaling Technology, Shanghai, China) and SIRT1 (1:1000; Abcam).

Wu J., Liang W., Tian Y., Ma F., Huang W., Jia Y., Jiang Z, & Wu H. (2019). Inhibition of P53/miR‐34a improves diabetic endothelial dysfunction via activation of SIRT1. Journal of Cellular and Molecular Medicine, 23(5), 3538-3548.

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Immunohistochemical Staining of Arterial Wall

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Immunohistochemical staining was performed as previously described,26 using antibodies against ac‐P53 (1:100; Abcam, Shanghai, China), SIRT1 (1:100; Abcam), vascular cell adhesion molecule‐1 (VCAM‐1, 1:100; Santa Cruz Biotechnology, Dallas, TX) and 4‐hydroxynonenal (4‐HNE, 1:100; Alpha Diagnostic Int., San Antonio, TX). Immunohistochemical positive area was quantified within the full‐thickness of the artery wall. Selection of areas to photograph and scoring were done by people blind to the identity of the samples.

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Tenovin-6 and Chloroquine Modulate p53 Signaling

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The following regents were used in this study: Tenovin-6 (BSCC-37, Agave Pharm, Seattle, WA, USA) and chloroquine diphosphate (C6628, Sigma, St. Louis, MO, USA). Antibodies specific to the following proteins were used: β-actin (RM001V, Beijing Ray Antibody Biotech, Beijing, China), p53 (#2527, Cell Signaling Technology, Danvers, MA, USA), Ac-p53 (ab75754, Abcam, Cambridge, MA,USA), p-p53 (#9284, Cell Signaling Technology, Danvers, MA, USA), p21 (#2947, Cell Signaling Technology, Danvers, MA, USA), L3B (sc-376404, Santa Cruz, Dallas, TX, USA), and p62 (18420-1-AP, Proteintech Group, Inc., Chicago, IL, USA).

Ke X., Qin Q., Deng T., Liao Y, & Gao S.J. (2020). Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine. Cancers, 12(2), 365.

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Immunohistochemical Staining of Oxidative Stress Markers

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IHC staining was done as previously described (Wu et al. 2014) (link), using antibodies against 3-nitrotyrosine (3-NT, Millipore; 1:100), 4-hydroxynonenal (4-HNE, Alpha Diagnostic Int., San Antonio, TX, USA; 1:100), vascular cell adhesion molecule-1 (VCAM-1, Santa Cruz Biotechnology; 1:100), intercellular adhesion molecule-1 (ICAM-1, Santa Cruz Biotechnology; 1:100), SIRT1 (Abcam; 1:100) and acetylated P53 (Ac-P53, Abcam; 1:100). IHC-positive area was quantified within the full-thickness of the artery wall, including endothelium, tunica media and tunica externa. Selection of areas to photograph and scoring was done by people blind to the identity of the samples.

Wu H., Wu J., Zhou S., Huang W., Li Y., Zhang H., Wang J, & Jia Y. (2018). SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. The Journal of endocrinology, 237(1).

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