Structural analysis of the as-synthesized Ag NWs and Ni(OH)2 NSs was performed using an X-ray diffractometer with Cu Kα radiation (λ = 0.1541 nm). The microstructures were observed by transmission electron microscopy (TEM, FEI Tecnai G2) and scanning electron microscopy (SEM, FEI Nova NanoSEM 450). The sheet thickness of Ni(OH)2 was measured by atomic force microscopy (AFM, Bruker) using a Scanasyst probe. The chemical bonding states were determined by X-ray photoelectron spectroscopy (XPS, ESCALAB250Xi spectrometer). The transmittance of the TEs was determined using a PerkinElmer UV-visible spectrometer. The Rs values were determined using a Four-point Probe Resistance Tester (Zhuhai Kaivo Optoelectronic Technology Co., Ltd.). The electrochemical performance was investigated using the electrochemical workstation (Autolab PGSTAT302 N).
Uv visible spectrometer
Manufactured by PerkinElmer
Sourced in United States
The UV–visible spectrometer is a laboratory instrument used to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is a core analytical tool for quantitative and qualitative analysis of various samples.
Automatically generated - may contain errors
Lab products found in correlation
Nova nanosem 450, by Thermo Fisher Scientific (1 mentions)
Scanasyst probe, by Bruker (1 mentions)
Tecnai g2, by Thermo Fisher Scientific (1 mentions)
Nano s90, by Malvern Panalytical (1 mentions)
Ir prestige, by Shimadzu (1 mentions)
Ics 3000, by Thermo Fisher Scientific (1 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
9 protocols using uv visible spectrometer
1
Structural and Electrochemical Analysis of Ag NWs and Ni(OH)2 NSs
2
Characterization of Biogenic Silver Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The absorption spectra of the prepared
nanoparticles mixture were
recorded by a PerkinElmer UV–visible spectrometer at regular
intervals by scanning the reacting mixture from 200 to 800 nm. The
Rigaku Ultima-III X-ray diffractometer was used to analyze the crystalline
nature of dried biogenic Ag nanoparticles. An IR-Prestige (Shimadzu)
Fourier transform infrared (FTIR) spectrometer was used to record
the FTIR spectra of biogenic Ag nanoparticles. For the transmission
electron microscopy (TEM) analysis, high-resolution JEOL-2010 TEM
(operating voltage is 200 kV) was used. The zeta potential of AgNPs
was recorded using water as a solvent and Zetasizer Nano S90 (Malvern)
at room temperature.
nanoparticles mixture were
recorded by a PerkinElmer UV–visible spectrometer at regular
intervals by scanning the reacting mixture from 200 to 800 nm. The
Rigaku Ultima-III X-ray diffractometer was used to analyze the crystalline
nature of dried biogenic Ag nanoparticles. An IR-Prestige (Shimadzu)
Fourier transform infrared (FTIR) spectrometer was used to record
the FTIR spectra of biogenic Ag nanoparticles. For the transmission
electron microscopy (TEM) analysis, high-resolution JEOL-2010 TEM
(operating voltage is 200 kV) was used. The zeta potential of AgNPs
was recorded using water as a solvent and Zetasizer Nano S90 (Malvern)
at room temperature.
3
AgNPs Catalyze Dye Reduction Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AgNPs
were used to catalyze the reduction of the dyes MO and PNP. A 1 mM
aqueous solution was prepared to catalyze PNP in DI water. The reaction
vessel was a UV quartz cuvette. After a quartz cuvette was put in,
the UV–visible spectra of 3 mL of PNP solution were obtained.
Then added 0.5 mL of freshly prepared 0.5 M concentration NaBH4 solution. The solution color changed from bright yellow to
dark yellow. In the quartz cuvette containing the solution mixture,
10 mg of Ag catalyst was added. Reduction began when the nanoparticles
were added to the cuvette, and spectra were recorded using a UV–visible
spectrometer between 200 and 800 nm (PerkinElmer). For MO dye, 0.04
mM concentration was used, following the same experimental procedure.
The catalytic proficiency of the catalysts was determined from
UV–visible spectra with the following equation where A0 is the
initial absorbance (at λmax 404 nm for PNP and 462
nm for MO), and At is the absorbance at
different time intervals (t).
were used to catalyze the reduction of the dyes MO and PNP. A 1 mM
aqueous solution was prepared to catalyze PNP in DI water. The reaction
vessel was a UV quartz cuvette. After a quartz cuvette was put in,
the UV–visible spectra of 3 mL of PNP solution were obtained.
Then added 0.5 mL of freshly prepared 0.5 M concentration NaBH4 solution. The solution color changed from bright yellow to
dark yellow. In the quartz cuvette containing the solution mixture,
10 mg of Ag catalyst was added. Reduction began when the nanoparticles
were added to the cuvette, and spectra were recorded using a UV–visible
spectrometer between 200 and 800 nm (PerkinElmer). For MO dye, 0.04
mM concentration was used, following the same experimental procedure.
The catalytic proficiency of the catalysts was determined from
UV–visible spectra with the following equation where A0 is the
initial absorbance (at λmax 404 nm for PNP and 462
nm for MO), and At is the absorbance at
different time intervals (t).
4
Quantifying Irradiation-Induced Analyte Release
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
During irradiation of HFs, liquid samples were collected from the soaking medium. Fluorine concentration was analyzed by ion-exchange chromatography on a Dionex ICS 3000. Titanium concentration was analyzed by inductively coupled plasma atomic emission spectroscopy (ICP-AES) on a Horiba Ultima machine (Kyoto, Japan). MB concentration was analyzed by absorption spectroscopy on a Perkin Elmer UV-visible spectrometer (Waltham, MA, USA) at 670 nm after diluting the samples in order to obtain an absorption value between 0 and 2. The Beer–Lambert law was used to link the absorbance to the concentration. A calibration curve was produced with MB solutions of concentrations ranging between 1.3 × 10−5 mol/L and 1.3 × 10−6 mol/L, giving a linear equation of A = 63441 C and a determination coefficient R2 of 0.999.
5
In Vitro Drug Release from Protein Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to estimate the release of the drug from the protein nanoparticles dialysis was done on ATV-BSA nanoparticles at pH 7.4 using PBS solution. The nanoformulation was placed in the dialysis bag in 5 mL PBS maintained at room temperature under constant stirring conditions. Equal volumes of PBS were withdrawn at predefined intervals of time, and the medium was replaced with the same volume of PBS. This study was carried out for about 80 hrs, and the concentration of drug released was estimated by determining the absorbance of the drug at 265 nm using a Perkin Elmer UV visible spectrometer (Lambda 25).
6
Comprehensive Material Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All fluorescence measurements
were done on a Jobin Yvon Fluoromax-4 spectrofluorometer. Powder X-ray
diffraction patterns were recorded on a Bruker D8 Advanced X-ray diffractometer
using Cu Kα radiation (λ = 1.5406 Å) in the 5–40°
2θ range. The IR spectra were acquired using a NICOLET 6700
FT-IR spectrophotometer using KBr pellet in the 400–4000 cm–1 range. Solid-state UV–visible spectra were
recorded on a PerkinElmer UV–visible spectrometer. Gas adsorption
measurements were studied using a BELSORP-max instrument from Bel
Japan. The SEM images were obtained using an FEI Quanta three-dimensional
dual beam Fourier transform scanning electron microscope at 30 kV.
were done on a Jobin Yvon Fluoromax-4 spectrofluorometer. Powder X-ray
diffraction patterns were recorded on a Bruker D8 Advanced X-ray diffractometer
using Cu Kα radiation (λ = 1.5406 Å) in the 5–40°
2θ range. The IR spectra were acquired using a NICOLET 6700
FT-IR spectrophotometer using KBr pellet in the 400–4000 cm–1 range. Solid-state UV–visible spectra were
recorded on a PerkinElmer UV–visible spectrometer. Gas adsorption
measurements were studied using a BELSORP-max instrument from Bel
Japan. The SEM images were obtained using an FEI Quanta three-dimensional
dual beam Fourier transform scanning electron microscope at 30 kV.
7
Optical Absorbance Measurements of Powders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For optical absorbance measurements,
powders were dispersed in an ethanol/hexane (1:3) solution. Absorbance
spectra were then collected with a PerkinElmer UV/visible spectrometer,
applying a background correction (blank) for the neat solvent mixture.
powders were dispersed in an ethanol/hexane (1:3) solution. Absorbance
spectra were then collected with a PerkinElmer UV/visible spectrometer,
applying a background correction (blank) for the neat solvent mixture.
8
Synthesis and Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCl (7647-01-0), CH3COOC2H5 (141-78-6), CH2Cl2(75-09-2) are acquired from Sd fine, India, 2-Hydroxy acetophenone (118-93-4), Na2SO4(239,313), H2SO4 (7664-93-9), Hexane (110-54-3), HNO3(7697-37-2), are purchased from Mark India, and L-Glutamine–(J60573.A1), Streptomycin (15,465,739), are purchased from Alfa Aesar. The given melting points were calculated in open capillaries using the Stuart melting point equipment at °C and are uncorrected. The UV spectra were recorded using a Systronics UV–visible spectrometer, while the IR spectra were recorded in cm−1 using a Perkin-Elmer Spectrum BX-I Infrared Spectrophotometer utilizing the KBr pellet. The Jeol JNM-ECS400 model instrument is used to capture the NMR data at 400 MHz, with TMS serving as an external reference. Iodine vapour is utilized to see the sample spots on the Silica gel-G coated glass plates, which are used to verify the purity of the compounds mentioned.
9
Hydrogel-Based Promethazine Drug Loading
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The loading of promethazine drug was done by weighing a known weight (2 g) of the hydrogel and immersed into the solution containers for adsorption to take place for 24 hours (equilibrium swelling) after which the hydrogel was removed from the solution and dried at room temperature for 48 hours. The residual concentration of the solution was measured to determine the percentage of drug entrapment in the hydrogel using Perkin Elmer UV-Visible Spectrometer at a wavelength of 254 nm. The percentage drug entrapment was calculated using the following equation.
Where Ci and Cf are the initial and final concentration of the drug respectively (Muhammad et al., 2016)
Where Ci and Cf are the initial and final concentration of the drug respectively (Muhammad et al., 2016)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!