FS-3 and BrP-LPA were purchased from Echelon Biosciences (Salt Lake City, UT). Anti-FLAG M2 (F1804) and anti-PDI (P7372) antibodies as well as FIPI were purchased from Sigma-Aldrich (St. Louis, MO), anti-GDE7 antibody (HPA041148) was from Atlas Antibodies (Stockholm, Sweden), anti-GAPDH antibody (M171-3) was from MBL (Tokyo, Japan), anti-tubulin β-3 antibody (921001) was from BioLegend (San Diego, CA). Anti-PPARγ (#2435), anti-adiponectin (#2789), anti-CD36 (#28109), and anti-FABP4 (#2120) antibodies were from Cell Signaling Technology (Danvers, MA). [14C]LPC (1-[14C]oleoyl-sn-glycerophosphocholine) was purchased from American Radiolabeled Chemicals (ARC 3094; St. Louis, MO). LPC (1-oleoyl-sn-glycerophosphocholine) and cPA (1-oleoyl-sn-glycero-2,3-cyclic-phosphate) were from Avanti Polar Lipids (Alabaster, AL). Nonidet P-40 and proK were procured from Nacalai Tesque (Kyoto, Japan). CHAPS was purchased from Dojindo (Kumamoto, Japan). BML 279 was purchased from Abcam (Cambridge, UK). S32826 was procured from Cayman Chemical (Ann Arbor, MI).
S32826
Manufactured by Cayman Chemical
Sourced in United States
S32826 is a laboratory instrument designed for the separation and purification of chemical compounds. It utilizes a chromatographic technique to achieve this function. The core purpose of this product is to facilitate the isolation and analysis of substances in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using s32826
1
Adipocyte Lipid Signaling Pathway
2
Antibody and Reagent Characterization for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ten kinds of antibodies and antisera were previously described [16 (link), 22 (link), 26 (link)] and summarized in Supplementary Table 1 . LPA (1-oleoyl-sn-3-glycerophosphate) and S32826 (a chemical inhibitor of ATX) were purchased from Cayman Chemical Co. (Ann Arbor, MI,
U.S.A.). Equine chorionic gonadotropin (eCG) and human CG (hCG) were purchased from Shionogi (Osaka, Japan) and Daiichi-Sankyo (Tokyo, Japan), respectively. The Vectastain Elite ABC staining kit for immunohistochemistry
was purchased from Vector Laboratories (Burlingame, CA, U.S.A.). Other standard reagents were of analytical grade.
U.S.A.). Equine chorionic gonadotropin (eCG) and human CG (hCG) were purchased from Shionogi (Osaka, Japan) and Daiichi-Sankyo (Tokyo, Japan), respectively. The Vectastain Elite ABC staining kit for immunohistochemistry
was purchased from Vector Laboratories (Burlingame, CA, U.S.A.). Other standard reagents were of analytical grade.
3
Quantification of Lipid Signaling Molecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FS-3, BrP-LPA (a mixture of two diastereomers), and oleoyl 3-ccPA were purchased from Echelon Biosciences (Salt Lake City, UT). Edelfosine (2-O-methyl PAF C-18) and S32826 were procured from Cayman Chemical (Ann Arbor, MI) and fluorescein from Tokyo Chemical Industry (Tokyo, Japan). Anti-FLAG M2 Ab (F1804) was purchased from Sigma-Aldrich (St. Louis, MO), anti-GDE4 Ab (27861-1-AP) from Proteintech (Rosemont, IL), anti-GDE7 Ab (HPA041148) from Atlas Antibodies (Stockholm, Sweden), anti-GAPDH Ab (M171-3) from MBL (Tokyo, Japan), and anti-Myc-Tag Ab (2276S) from Cell Signaling Technology (Danvers, MA). We obtained 1-oleoyl-LPC and 1-oleoyl-lysophosphatidylethanolamine (LPE) from Avanti Polar Lipids (Alabaster, AL) and DTT from Cytiva-GE Healthcare (Marlborough, MA). Nonidet P-40 (NP-40) was procured from Nacalai Tesque (Kyoto, Japan). 1-[14C]Oleoyl-LPC was enzymatically prepared from [14C]dioleoylphosphatidylcholine (American Radiolabeled Chemicals, St. Louis, MO) using Naja mossambica mossambica phospholipase A2 (Sigma-Aldrich) and purified by TLC.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!