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Anti mouse caspase 1

Manufactured by Abcam
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Anti-mouse caspase-1 is a laboratory reagent used for the detection and quantification of caspase-1 in mouse samples. Caspase-1 is an enzyme involved in the inflammatory response and apoptosis. This product can be used in various research applications that require the measurement of caspase-1 activity or levels in mouse models.

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Lab products found in correlation

Anti β actin, by Merck Group (3 mentions) Anti mouse il 1β, by R&D Systems (3 mentions) Anti mouse nlrp3, by Cell Signaling Technology (3 mentions) Anti human il 1β, by R&D Systems (2 mentions) Anti human caspase 1, by Abcam (2 mentions) Anti human nlrp3, by Abcam (2 mentions) Enhanced chemiluminescence reagent, by Cytiva (1 mentions)

Spelling variants of this product

Caspase-1 (146 citations) Anti-caspase-1 (72 citations) Mouse anti (2 citations)

4 protocols using anti mouse caspase 1

1

Inflammatory Pathway Activation in Viral Lung Infection

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Lungs were harvested one day post-infection, dissolved in lysis buffer and homogenized for Western blot assay using anti-mouse IL-1β (R&D Systems), anti-mouse caspase-1 (Abcam, Cambridge, MA), anti-mouse NLRP3 (Cell Signaling Technology), and anti-β actin (Millipore Sigma, Burlington, MA). RV-A1B- and RV-C15-infected THP-1-derived macrophages were harvested one-day post-infection, dissolved in lysis buffer for Western blot assay using anti-human IL-1β (R&D Systems), anti-human caspase-1 (Abcam), anti-human NLRP3 (Abcam).
Han M., Ishikawa T., Stroupe C.C., Breckenridge H.A., Bentley J.K, & Hershenson M.B. (2021). Deficient inflammasome activation permits an exaggerated asthma phenotype in rhinovirus C-infected immature mice. Mucosal immunology, 14(6), 1369-1380.
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2

Inflammatory Pathway Activation in Viral Lung Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lungs were harvested one day post-infection, dissolved in lysis buffer and homogenized for Western blot assay using anti-mouse IL-1β (R&D Systems), anti-mouse caspase-1 (Abcam, Cambridge, MA), anti-mouse NLRP3 (Cell Signaling Technology), and anti-β actin (Millipore Sigma, Burlington, MA). RV-A1B- and RV-C15-infected THP-1-derived macrophages were harvested one-day post-infection, dissolved in lysis buffer for Western blot assay using anti-human IL-1β (R&D Systems), anti-human caspase-1 (Abcam), anti-human NLRP3 (Abcam).
Han M., Ishikawa T., Stroupe C.C., Breckenridge H.A., Bentley J.K, & Hershenson M.B. (2021). Deficient inflammasome activation permits an exaggerated asthma phenotype in rhinovirus C-infected immature mice. Mucosal immunology, 14(6), 1369-1380.
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3

Immunoblotting of NLRP3 and Caspase-1

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A 10 μg of protein from lung tissue sample was electrophoresed on 15% polyacrylamide gels using a discontinuous system. The proteins were transferred to nitrocellulose membranes at 120 V for 40 minutes. After blocking in 5% skim milk and 0.1% NP40 in TBS for 2 hours at room temperature, the membranes were incubated with a rabbit polyclonal anti-mouse NLRP3-inflammasome (Abnova) or rabbit monoclonal anti-mouse caspase-1 (Abcam, Cambridge, MA, USA) primary antibody. The membrane was incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (1:2,000 dilution) for 1 hour at room temperature. The target protein was detected using an enhanced chemiluminescence reagent (Amersham Pharmacia Biotech, Little Chalfont, UK).
Uh S.T., Koo S.M., Kim Y., Kim K., Park S., Jang A.S., Kim D., Kim Y.H, & Park C.S. (2017). The activation of NLRP3-inflammsome by stimulation of diesel exhaust particles in lung tissues from emphysema model and RAW 264.7 cell line. The Korean Journal of Internal Medicine, 32(5), 865-874.
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4

Lung Inflammation Protein Analysis

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Lungs were harvested one day post-infection, dissolved in lysis buffer and homogenized for Western blot assay using anti-mouse IL-1β (R&D Systems), anti-mouse caspase-1 (Abcam, Cambridge, MA), anti-mouse NLRP3 (Cell Signaling Technology), and anti-β actin (Millipore Sigma, Burlington, MA).
Han M., Ishikawa T., Bermick J.R., Rajput C., Lei J., Goldsmith A.M., Jarman C.R., Lee J., Bentley J.K, & Hershenson M.B. (2020). IL-1β prevents ILC2 expansion, type 2 cytokine secretion and mucus metaplasia in response to early-life rhinovirus infection in mice. Allergy, 75(8), 2005-2019.
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