The cells were collected by a cell scraper (Corning) with cool lysate (Beyotime) for protein extraction. The lysate was incubated with anti‐Flag antibodies (F1804; Merck, Darmstadt, Germany) or an equal amount of mouse IgG (B900620; Proteintech) on a rotating wheel overnight at 4°C and then incubated with protein A agarose beads (Beyotime) at 4°C for 10 h. Beads were collected by centrifugation, washed, boiled in 2 × PAGE loading buffers (Beyotime) and analysed by western blotting. The experiments were repeated three times.
Anti flag antibodies f1804
Manufactured by Merck Group
Sourced in Germany
Anti‐Flag antibodies (F1804) are laboratory reagents used to detect and purify proteins that have been engineered to contain a specific peptide tag called the Flag tag. The antibodies bind to this tag, allowing researchers to identify and isolate the tagged proteins for further analysis.
Automatically generated - may contain errors
2 protocols using anti flag antibodies f1804
1
Flag-tagged Protein Immunoprecipitation
2
Immunoprecipitation and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells were collected by a cell scraper (Corning, USA) with cool lysate (Beyotime, China) for protein extraction. The lysate was incubated with anti‐Flag antibodies (F1804, Merck, Germany) or an equal amount of mouse IgG (B900620, Proteintech, China) on a rotating wheel overnight at 4°C and then incubated with protein A agarose beads (Beyotime, China) at 4°C for 10 h. Beads were collected by centrifugation, washed, boiled in 2× PAGE loading buffers (Beyotime, China) and analysed by Western blotting. The experiments were repeated three times.
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