Bks cg m leprdb j mice

Male 7 weeks-old BKS.Cg-m +/+ Lepr^db/J mice (db/db: T2D) and control C57BLKS/J wild-type mice were purchased from The Jackson Laboratory (Bar Harbor, ME). In a subgroup of wild-type mice, T1D was induced after one week (8 weeks-old) with intraperitoneal injections of streptozotocin (Sigma-Aldrich, St. Louis, MO) 50 mg kg⁻¹ day⁻¹ during 10 days. The animals were housed under a constant 12 h dark/light cycle and were given standard chow and water ad libitum. At 12 weeks-old, blood samples were collected via the submandibular vein and the animals were sacrificed by cervical dislocation. Livers and small intestine were quickly harvested, washed with ice-cold phosphate-buffered saline (PBS), snap-frozen in liquid nitrogen and kept at −80°C. These experiments were carried out in accordance with the Declaration of Helsinki and with the Guide to the Care and Use of Experimental Animals of the Canadian Council on Animal Care.