Log In Sign up
The largest database of trusted experimental protocols

Biospot 5

Manufactured by Cellular Technology
Visit Supplier

BioSpot 5.0 is a software application designed for the analysis and quantification of biological samples. The software provides tools for image acquisition, processing, and data analysis. BioSpot 5.0 is compatible with a range of laboratory equipment and can be used for various applications in the field of biotechnology and life sciences.

Automatically generated - may contain errors

Lab products found in correlation

Automated counting system, by Cellular Technology (2 mentions) Diaminobenzidine staining abc kit, by Vector Laboratories (2 mentions)

Close spelling variants of this product

Biospot Version 5.0 software

2 protocols using biospot 5

1

Neutralizing Antibody Assay for HCVcc 1a/2a Chimera

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCVcc 1a(H77)/2a chimeric virus was generated as previously described.36 The E1E2 region was based on the H77 consensus sequence (accession no.: AF009606). This region (aa 192‐746 of the HCV polyprotein) shares 94.8% homology with the genotype 1a E1E2 sequence used for the phase I vaccine study (HCV‐1; accession no.: M62321; http://onlinelibrary.wiley.com/doi/10.1002/hep.28108/suppinfo). Neutralizing antibody titers were determined using Huh7.5 cells by performing 2‐fold dilutions of sera from 1:32 to 1:1,024 and mixing 1:1 with HCVcc 1a/2a (100 focus forming units [ffu]/50 μL), as previously described,36 to give starting dilutions of 1:64. After 72 hours, cells were fixed (2% formaldehyde), stained with anti‐HCV core mouse mAb (6G7; diluted 1:1,000), and developed with the Diaminobenzidine‐staining ABC Kit (Vector Laboratories, Burlingame, CA), as previously described.37 Foci were counted using an automated counting system (Cellular Technology Limited [Cleveland, OH] using BioSpot 5.0 software).
Kachko A., Frey S.E., Sirota L., Ray R., Wells F., Zubkova I., Zhang P, & Major M.E. (2015). Antibodies to an interfering epitope in hepatitis C virus E2 can mask vaccine‐induced neutralizing activity. Hepatology (Baltimore, Md.), 62(6), 1670-1682.
+ Open protocol
+ Expand
2

Generation and Neutralization of HCV Chimeric Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCVcc 1a(H77)/2a chimeric virus was generated as previously described (36 (link)). The E1E2 region was based on the H77 consensus sequence (Accession number AF009606). This region (aa 192–746 of the HCV polyprotein) shares 94.8% homology with the Genotype 1a E1E2 sequence used for the Phase I vaccine study (HCV-1, Accession number M62321) (Supplemental Figure S1). Neutralizing antibody titers were determined using Huh7.5 cells by performing 2-fold dilutions of sera from 1:32 to 1:1024 and mixing 1:1 with HCVcc 1a/2a (100 ffu/50μl) as previously described (36 (link)) to give starting dilutions of 1:64. After 72 hours cells were fixed (2% formaldehyde), stained with anti-HCV core mouse monoclonal antibody (6G7) (diluted 1:1000) and developed with Diaminobenzidine-staining ABCKit (Vector Laboratories, Burlingame, CA) as previously described (37 (link)). Foci were counted using an automated counting system (Cellular Technology Limited using BioSpot 5.0 software).
Kachko A., Frey S.E., Sirota L., Ray R., Wells F., Zubkova I., Zhang P, & Major M.E. (2015). Antibodies to an interfering epitope in hepatitis C virus E2 can mask vaccine‐induced neutralizing activity. Hepatology (Baltimore, Md.), 62(6), 1670-1682.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!