In situ cell death detection kit tmr red version 11

As described previously (Qi and Calvi 2016 (link)), young adult females were conditioned on wet yeast for three days and then exposed to 40 Gy of gamma irradiation from a cesium source. Four hours after irradiation, ovaries were dissected and stained by the TUNEL method (In Situ Cell Death Detection Kit, TMR red, version 11 (12 156 792 910; Roche, Basel, Switzerland)) and DAPI. Percent of apoptotic cells was determined by scoring TUNEL staining of cells with DAPI-stained nuclei in flattened follicle surfaces, excluding deformed cells at follicle edges and the polar follicle cells that have been shown previously to undergo p53-independent, developmentally programmed cell death (Besse and Pret 2003 (link); Mehrotra et al. 2008 (link)).