Reporter plasmids

Manufactured by Promega

Sourced in United States

Reporter plasmids are genetic constructs that contain a reporter gene, such as luciferase or fluorescent proteins, under the control of a promoter or regulatory sequences. These plasmids are used to monitor and quantify gene expression in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Fh535, by Merck Group (2 mentions) One glo reagent, by Promega (1 mentions)

Close spelling variants of this product

PGL3-promoter reporter plasmids PmirGLO-based luciferase reporter plasmids Firefly reporter pGL4.75 plasmids Dual-luciferase reporter gene plasmids Renilla luciferase reporter control plasmid Luciferase reporter gene plasmid PMIR-REPORT luciferase reporter plasmids TK-Renilla reporter plasmid Dual-luciferase reporter plasmid PGL4.14 reporter plasmid

3 protocols using reporter plasmids

Luciferase Reporter Assay for NF-κB and SMAD

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Reporter plasmids (Promega, Madison, WI, USA) with luciferase driven by NFκB or SMAD responsive elements were stably established in HEK293A cells and maintained by a puromycin-containing culture medium. Chemiluminescence levels represent the luciferase expression was determined by the ONE-Glo reagent (Promega, USA). The fold change of each group to the DMSO vehicle control group was calculated.

Wang H.C., Ke T.Y., Ko Y.C., Lin J.J., Chang J.S, & Cheng Y.B. (2021). Anti-Inflammatory Azaphilones from the Edible Alga-Derived Fungus Penicillium sclerotiorum. Marine Drugs, 19(10), 529.

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Analyzing Wnt Signaling in VSMC

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Effects of Pi on Wnt signaling in VSMC were analyzed using a luminescent reporter plasmid assay. Briefly, VSMC were transiently transfected with reporter plasmids (Promega, Mannheim, Germany) for the T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) binding motif (pGL4.49[luc2P/TCF-LEF RE/Hygro]) and a renilla vector (pGL4.74[hRluc/TK]). To inhibit Wnt activation, the β-Catenin/TCF site-inhibitor FH535 (10 µM; Merck) was used.

Freise C., Biskup K., Blanchard V., Schnorr J, & Taupitz M. (2024). Inorganic Phosphate-Induced Extracellular Vesicles from Vascular Smooth Muscle Cells Contain Elevated Levels of Hyaluronic Acid, Which Enhance Their Interaction with Very Small Superparamagnetic Iron Oxide Particles. International Journal of Molecular Sciences, 25(5), 2571.

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Wnt Signaling Pathway Modulation in VSMCs

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VSMC were transiently transfected with reporter plasmids (Promega, Mannheim, Germany) for the T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) binding motif (pGL4.49[luc2P/TCF-LEF RE/Hygro]) and a renilla vector (pGL4.74[hRluc/TK]) as described [18 (link)]. As a positive control for Wnt activation the Wnt agonist I (CAS 853220-52-7; Merck, Schwalbach, Germany) was used. To inhibit Wnt activation, the β-Catenin/TCF site-inhibitor FH535 (10 μM; Merck) was used.

Freise C., Kretzschmar N, & Querfeld U. (2016). Wnt signaling contributes to vascular calcification by induction of matrix metalloproteinases. BMC Cardiovascular Disorders, 16, 185.

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