GzmctdT-iCreRosa26LSL-STAT5b-CA, GzmctdT-iCreRosa26LSL-STAT5b-CAPerf−/−, or wild-type littermate mice were euthanized at 14 days of age with CO2. Following gross examination the liver was fixed in 10% neutral buffered formalin, followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor. Paraffin blocks were sectioned at 5 microns, stained with hematoxylin and eosin (H&E), and examined by a board-certified veterinary pathologist (AOM).
Asp6025 tissue processor
Manufactured by Leica
Sourced in Germany
The ASP6025 is a tissue processor designed for automated tissue preparation. It is capable of processing up to 75 tissue samples simultaneously through a series of dehydration, clearing, and infiltration steps. The device features a compact design and user-friendly interface for efficient tissue processing.
Automatically generated - may contain errors
5 protocols using asp6025 tissue processor
1
Liver Histopathology of Genetically Modified Mice
2
Comprehensive Histological Evaluation of Mouse Organs
3
Histopathological Analysis of Adam17 Mutant Mice
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The histopathological analysis of adult mice was performed on three pairs of mice from independent litters on a mixed 129SvJ/C57Bl/6J-TyrC-2J genetic background, each pair consisting of one Adam17Δcyto mutant and one wild-type littermate control. Two pairs were females and one pair was male. All mice were approximately 6.5 weeks old.
Mice were euthanized with CO2. Following gross examination, all organs were fixed in 10% neutral buffered formalin, followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor. Paraffin blocks were sectioned at 5 microns, stained with hematoxylin and eosin (H&E), and examined by a board-certified veterinary pathologist. The following tissues were processed and examined: the heart, thymus, lungs, liver, gallbladder, kidneys, pancreas, stomach, duodenum, jejunum, ileum, cecum, colon, lymph nodes (submandibular, mesenteric), salivary glands, skin (trunk and head), urinary bladder, uterus, cervix, vagina, ovaries, oviducts, adrenal glands, spleen, thyroid gland, esophagus, trachea, spinal cord, vertebrae, sternum, femur, tibia, stifle join, skeletal muscle, nerves, skull, nasal cavity, oral cavity, teeth, ears, eyes, pituitary gland, brain.
Mice were euthanized with CO2. Following gross examination, all organs were fixed in 10% neutral buffered formalin, followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor. Paraffin blocks were sectioned at 5 microns, stained with hematoxylin and eosin (H&E), and examined by a board-certified veterinary pathologist. The following tissues were processed and examined: the heart, thymus, lungs, liver, gallbladder, kidneys, pancreas, stomach, duodenum, jejunum, ileum, cecum, colon, lymph nodes (submandibular, mesenteric), salivary glands, skin (trunk and head), urinary bladder, uterus, cervix, vagina, ovaries, oviducts, adrenal glands, spleen, thyroid gland, esophagus, trachea, spinal cord, vertebrae, sternum, femur, tibia, stifle join, skeletal muscle, nerves, skull, nasal cavity, oral cavity, teeth, ears, eyes, pituitary gland, brain.
4
Rat Cornea Histological Examination
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The rat corneas were fixed with 10% formaldehyde neutral buffer solution (Nacalai Tesque) and paraffin (Thermo Fisher Scientific) using an ASP6025 Tissue Processor (Leica, Wetzlar, Germany). Samples were cut into 8 µm sections using a SM2010R microtome (Leica) and stained with H&E (Sakura Finetek Japan, Tokyo, Japan). Subsequently, the sections were imaged using an Axio Observer Z1, D1 (Carl Zeiss).
5
Comprehensive Histopathological Analysis of Mice
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Mice were euthanized with CO2. Following gross examination, all organs were fixed in 10% neutral buffered formalin, followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor. Paraffin blocks were sectioned at 5 µm, stained with hematoxylin and eosin (H&E), and examined by a board-certified veterinary pathologist. The following tissues were processed and examined: heart, thymus, lungs, liver, gallbladder, kidneys, pancreas, stomach, duodenum, jejunum, ileum, cecum, colon, lymph nodes (submandibular, mesenteric), salivary glands, skin (trunk and head), urinary bladder, uterus, cervix, vagina, ovaries, oviducts, adrenal glands, spleen, thyroid gland, esophagus, trachea, spinal cord, vertebrae, sternum, femur, tibia, stifle join, skeletal muscle, nerves, skull, nasal cavity, oral cavity, teeth, ears, eyes, pituitary gland, and brain. To detect goblet cells in the intestine, the AB/PAS kit (Thermo Fisher #87023) was used according to the manufacturer’s instructions.
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