Hyclone bovine serum

Manufactured by GE Healthcare

Hyclone bovine serum is a high-quality animal-derived cell culture supplement used to support the growth and proliferation of cells in in vitro experiments. It provides essential nutrients, growth factors, and other components necessary for maintaining cell health and optimal cell culture performance.

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Lab products found in correlation

Penicillin and streptomycin, by Corning (2 mentions) Gentamicin sulfate, by Corning (2 mentions) Medium 199, by Corning (2 mentions) Hyclone 2 mm l glutamine, by GE Healthcare (2 mentions) Dmem media, by Lonza (2 mentions)

2 protocols using hyclone bovine serum

Culturing Human and Murine Cells for Toxoplasma Assays

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Human foreskin fibroblasts (HFF) and murine macrophages were obtained from American Type Culture Collection (ATCC). All cell lines and parasite strains were maintained in D10 media which consisted of DMEM media (Lonza) supplemented with 10% heat inactivated Hyclone bovine serum (GE Healthcare Life Sciences), HyClone 2 mM L-glutamine (GE Healthcare Life Sciences), 100 μg/mL penicillin and streptomycin (Corning), 20% Medium 199 (Corning) and gentamicin sulfate (Corning) at 37°C with 5% CO₂. Type I strain of T. gondii constitutively expressing red fluorescent dimerized Tomato (“RH-dTom”) and a type II strain, PRU expressing the same fluorophore (“PRU-dTom”) were used in assays.

Sanford A., Schulze T., Potluri L., Hemsley R., Larson J., Judge A., Zach S., Wang X., Charman S., Vennerstrom J, & Davis P. (2018). Novel Toxoplasma gondii Inhibitor Chemotypes. Parasitology international, 67(2), 107-111.

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Culturing Human Cell Lines and Toxoplasma Strains

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Human foreskin fibroblasts (HFF), human osteosarcoma cells (U-20S), human embryonic kidney cells (HEK-293), human liver cells (HC-04), and murine macrophages (NR-9456) were all obtained from ATCC. HFF, U-20S, and all T. gondii strains (RH-dTom, ME49 and PRU) used were maintained in DMEM media (Lonza) supplemented with 10% heat inactivated Hyclone bovine serum (GE Healthcare Life Sciences), HyClone 2 mM L-glutamine (GE Healthcare Life Sciences), 100 μg/mL penicillin and streptomycin (Corning), 10% Medium 199 (Corning) and gentamicin sulfate (Corning) at 36.5 °C with 5% CO₂. HEK-293 and HC-04 were maintained in the same medium without the addition of Medium 199. Macrophage NR-9456 was grown with the addition of 1 mM sodium pyruvate as recommended.

Sanford A.G., Schulze T.T., Potluri L.P., Watson G.F., Darner E.B., Zach S.J., Hemsley R.M., Wallick A.I., Warner R.C., Charman S.A., Wang X., Vennerstrom J.L, & Davis P.H. (2018). Derivatives of a benzoquinone acyl hydrazone with activity against Toxoplasma gondii. International Journal for Parasitology: Drugs and Drug Resistance, 8(3), 488-492.

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