After mice were sacrificed, their colons were Swiss-rolled, fixed in 4% formalin, and embedded in paraffin. Each paraffin block was sequentially sectioned at 4 μm, and the sections were mounted on slides. Immunohistochemical staining was performed using previously described method.20 (link) Briefly, the sections were rinsed and endogenous peroxidase activity was blocked with 3% hydrogen peroxide. The sections were then washed with PBS, blocked with M.O.MTM Mouse IgG blocking reagent (Vector Laboratories, Inc., Burlingame, CA, USA), and incubated overnight with diluted primary antibodies. The sections were washed with PBS and incubated with biotinylated secondary antibodies for 1 hr and immunostaining was visualized with a Vectastain ABC kit, using DAB as a substrate (Vector Laboratories, Inc.). The sections were rinsed, dehydrated, mounted, and covered with coverslips. The tissue slides were observed under a light microscope (Leica DM1000 LED; Leica Microsystems, Wetzlar, Germany) and images were captured using an LAS Image Analyzer (LAS ver 4.0; Leica Microsystems).
M o mtm mouse igg blocking reagent
Manufactured by Vector Laboratories
Sourced in United States
M.O.MTM Mouse IgG blocking reagent is a laboratory product designed to block non-specific binding of mouse immunoglobulin G (IgG) in immunoassays or other applications where mouse IgG may interfere with the analysis.
Automatically generated - may contain errors
Lab products found in correlation
Dm1000 led, by Leica (1 mentions)
Vectastain abc kit, by Vector Laboratories (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions)
Axiocam erc5, by Zeiss (1 mentions)
Slowfade diamond antifade reagent, by Thermo Fisher Scientific (1 mentions)
Tcs sp8, by Leica (1 mentions)
2 protocols using m o mtm mouse igg blocking reagent
1
Immunohistochemical Colon Tissue Analysis
2
Immunofluorescent Staining of Muscle Tissue
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Frozen transverse sections were cut at the thickness of 8 μm and fixed for 5 min in ice-cooled acetone. After blocking with M.O.M.TM mouse IgG blocking reagent (Vector Laboratories), sections were incubated overnight at 4°C with primary antibodies diluted in M.O.M.TM diluent. After washing with PBS, sections were stained with secondary antibodies. Primary and secondary antibodies used were listed in Supplementary Table 2 . Nuclei were counterstained with DAPI (Dojindo), and stained muscles were mounted with SlowFade Diamond anti-fade reagent (Invitrogen). Fluorescent signals were detected with confocal laser scanning microscope systems TCS-SP8 (Leica). The same sections were stained with hematoxylin and eosin (HE) after capturing fluorescent images. HE images were taken with microscope AXIO (Carl Zeiss) equipped with a digital camera, Axiocam ERc 5s (Carl Zeiss).
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