Human anti-PD-L1-Rb (ab213524) and mouse anti-PD-L1-Rb (ab213480) were purchased from Abcam; anti-CD8α-Rb (GB11068 and GB11068-1) was purchased from Servicebio; anti-PD-1-Rb (84651) was purchased from Cell Signaling Technology; the antibodies specific for human anti-MMP2-Rb (10375-2-AP), anti-MMP9-Rb (10373-2-AP), anti-MMP9-Rb (10373-2-AP), anti-GAPDH-M (60004-1-Ig), and anti-PD-L1-M (66248) were purchased from Proteintech; SB-3CT was purchased from Selleck(S7430); and in vivo mAb anti-PD-1-M (BE0146), anti-CTLA-4-M (BE0164), and IgG isotype control (BE0086, BE0089) were purchased from Bioxcell.
Gb11068 1
Manufactured by Wuhan Servicebio Technology
GB11068-1 is a laboratory equipment designed for scientific research and analysis. It serves as a versatile instrument to facilitate various laboratory procedures. The core function of this product is to provide a controlled environment and reliable data collection for scientific experiments and investigations. However, a more detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab213480, by Abcam (1 mentions)
Ab213524, by Abcam (1 mentions)
Sb 3ct, by Selleck Chemicals (1 mentions)
Be0086, by BioXCell (1 mentions)
Be0164, by BioXCell (1 mentions)
Gb14043, by Wuhan Servicebio Technology (1 mentions)
Image pro plus 6, by Leica camera (1 mentions)
Gb13064 1, by Wuhan Servicebio Technology (1 mentions)
Icc50w, by Leica camera (1 mentions)
Gb11064 1, by Wuhan Servicebio Technology (1 mentions)
Gb11014, by Wuhan Servicebio Technology (1 mentions)
Gb11064, by Wuhan Servicebio Technology (1 mentions)
5 protocols using gb11068 1
1
Immunohistochemical Analysis of Immune Checkpoint Proteins
2
Immunohistochemical Analysis of T-Cell and Macrophage Markers
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In short, samples were fixed in 4% paraformaldehyde, and embedded in paraffin for hematoxylin-eosin staining (H&E) or immunohistochemical analysis. Samples were incubated with CD4 (1:50, GB13064-1, Servicebio), CD8 (1:200, GB11068-1, Servicebio), and CD68 (1:200, GB14043, Servicebio) at 4 °C overnight and secondary antibody labeled with horseradish peroxidase at room temperature for 2 h. Finally, samples were counterstained with hematoxylin and visualized with 3,3’-diaminobenzidine (DAB) tetrahydrochloride for 10 min. All the sections were examined and the pictures were taken by digital camera (Leica, ICC50 W) and protein expressions were quantified by Image-Pro Plus 6.0.
3
Immunohistochemical Profiling of Immune Markers
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For IHC assay, paraffin sections were incubated with primary antibodies against CD8A (1:300; Servicebio, catlog#GB11068-1), PD-L1 (1:500, Servicebio, catlog#GB11339A) and CMTM6 (1:500, Abcam, catlog#ab264067) at 37°C for 60 mins, secondary antibodies at 37°C for 15 mins and horseradish enzyme labelled streptavidin solution for 10 mins, then stained by DAB and hematoxylin. Staining percentage scores were classified as follows: 1 (1%–25%), 2 (26%–50%), 3 (51%–75%) and 4 (76%–100%), and staining intensity was scored 0 (signalless color) to 3 (light yellow, brown, and dark brown). The stained tissues were scored by three individuals blinded to the clinical parameters, and the IHC scores were determined by percentage and intensity scores.
4
Immunohistochemical Analysis of SUPS Specimens
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Tissue sectioning and immunohistochemistry staining of formalin-fixed, paraffin-embedded SUPS specimens were performed. All sections were deparaffinized, rehydrated, and washed. Endogenous peroxidase was blocked using 3% hydrogen peroxide for 10 min. After water-bath heating for antigen retrieval, slides were incubated with primary antibodies followed by horseradish peroxidase (HRP)-linked secondary antibodies and diaminobenzidine staining (G1213-100UL, G1214-100UL). Hospital Pathology Department blinded to clinical data independently assessed staining results for SMA (unnecessary dilution, ZM-0003,ZSGB-BIO), CD68 (unnecessary dilution, ZM-0464, ZSGB-BIO), Ki67 (1:100, ZM-0166, ZSGB-BIO), CD34 (unnecessary dilution, ZM-0046, ZSGB-BIO), Desmin (unnecessary dilution, ZA-0686, ZSGB-BIO), EMA (unnecessary dilution, ZM-0095, ZSGB-BIO), S-100 (unnecessary dilution, ZM-0224, ZSGB-BIO), BCL-2 (unnecessary dilution, ZA-0536, ZSGB-BIO). Servicebio blinded to clinical data independently assessed staining results for CD3 (1:250, GB11014, Servicebio), CD4 (1:400, GB11064-1, Servicebio), CD8 (1:200, GB11068-1, Servicebio). Quantification was performed by counting positive cells in 6 to 10 high-powered fields (magnification, ×40) in a blinded fashion.
5
Immunohistochemical Analysis of Tumor Infiltration
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Tumor tissues and spleens resected from mice were fixed in 4% paraformaldehyde and then embedded in paraffin for subsequent sectioning. Each sample was cut into 4 μm sections and then incubated using mouse antihuman CD4 antibody (1:100 dilution; rabbit no. GB11068-1, SERVICEBIO) and anti-CD8 antibody (1:100 dilution; rabbit no. GB11064, SERVICEBIO) overnight at 4°C to analyze spleen infiltration, which was then further incubated using a rabbit antimouse antibody at room temperature for 2 h (1:1,000 dilution; rabbit no. SP-9001, Beijing Zhongshan Jinqiao Biological Company). Thereafter, the sections were stained using diaminobenzidine (K135925C, Beijing Zhongshan Jinqiao Biological Company) and hematoxylin. For approximately 2 min, the sections were stained using a DAB color development kit.
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