Nexion 300d dynamic reaction cell

Aqueous elemental analyses for this study were performed using inductively coupled optical emission spectrometry (ICP-OES) (Perkin Elmer Optima 5300DV) and inductively coupled plasma-mass spectrometry (ICP-MS) (Perkin Elmer NexION 300D-Dynamic Reaction Cell) in aqueous solutions for trace element content (As, Cr, Fe, Mn, and U). Samples for ICP-MS were filtered using a 0.45 μm Basix filter and preserved with 2% HNO₃⁻. A Thermo Fisher Scientific Ion Chromatogram (ICS-1100) was used to analyze 0.45 μm non-acidified water samples for major anion concentrations. Arsenic speciation was characterized using a Flexar 400 High-Performance Liquid Chromatography (HPLC) coupled with ICP-MS for As(III) and As(V) species. Samples were stabilized with a mobile phase solution (0.5 mM EDTA, 1 mM TBAoH and 1% MeOH) adjusted to pH 7 (±0.01). A CAPCELL PAK C18 column (250 mm × 4.6 μm, 5 μm particle size) was used for the separation of target As species. All reagents used in the experiments and analyses were analytical grade unless otherwise mentioned. Additional information about the HPLC-ICP-MS analyses can be found in ESI (Table S2†).

Aqueous elemental analyses for this study were performed using inductively coupled optical emission spectrometry (ICPOES) (PerkinElmer Optima 5300DV) and inductively coupled plasma-mass spectrometry (ICP-MS) (PerkinElmer NexION 300D-Dynamic Reaction Cell) in aqueous solutions for trace element content (As, Fe, and Mn). Samples for ICP-MS were filtered using a 0.45 μm Basix filter and preserved with 2% HNO₃. A Thermo Fisher Scientific Ion Chromatogram (ICS-1100) was used to analyze 0.45 μm filtered nonacidified water samples for major anion concentrations. Aqueous Fe(II) was measured by the ferrozine assay, and Fe(II) standards were prepared from ferrous ammonium sulfate (GFS Chemicals). All reagents used in the experiments and analyses were analytical grade. No unexpected or unusually high safety hazards were encountered.