Hiscript 2 q rt supermix for rt pcr

PFOS (potassium salt; >98% pure) was purchased from MAYA-R (Jiaxing, China). Dimethyl sulfoxide (DMSO) and the cell counting kit-8 (CCK-8) were purchased from Vicmed (Busan, Korea). MEM medium was purchased from Corning (Shanghai, China). DMEM/F12 medium and RPMI 1640 medium were purchased from KeyGEN BioTECH (Nanjing, China). Fetal bovine serum was purchased from ExCell Bio. HiScript^® II Q RT SuperMix for RT-PCR and AceQ^® RT-PCR were obtained from Vazyme (Nanjing, China). Rosiglitazone and GW9662 were purchased from MedChemExpress (Shanghai, China). pcDNA-PPARγ and siRNAs were obtained or synthesized by GenePharma (Shanghai, China). Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) was purchased from Invitrogen and used for transient transfection.

One milliliter of Trizol reagent (R401-01, Vazyme, Nanjing, China) was added to the target cells for lysis to obtain RNA. The HiScript^® II Q RT SuperMix for RT-PCR (+gDNA wiper, R223-01, Vazyme, Nanjing, China) was used to synthesize the first-strand cDNA. Quantitative PCR was performed using a real-time PCR System (CFX Connect, Bio-Rad, Hercules, CA, USA) with MonAmp™ ChemoHS qPCR Mix (MQ00401S, Monad, Shanghai, China). The primers for this experiment were derived from Sangon (Shanghai, China). Table S1 lists the primer sequences.