Phosphotau

Manufactured by Thermo Fisher Scientific

Sourced in United States

PhosphoTau is a laboratory instrument designed for the detection and quantification of phosphorylated tau proteins. It utilizes advanced technologies to analyze samples and provide accurate measurements of tau phosphorylation levels, which are important biomarkers for certain neurological conditions.

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Lab products found in correlation

2 protocols using phosphotau

Detailed Immunodetection Procedures

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The following primary antibodies were used for the described experiments: NeuN (Millipore Mab377 1:300), NMDAR2B (Cell Signaling 4212S 1:2000), NR2A (Cell Signaling 4205S 1:500), GluR1 (Abcam ab7260 1:2000), GluR2/3 (Millipore 07-598 1:2000), β-actin (Abcam ab8227 1:3000), G10 ((70 (link)) 1:1000 for western blotting, 1:500 for immunohistochemistry), 6E10 (Covance SIG-39320), 4G8 (Covance SIG-39220), Dab1 (5091 (71 (link)) 1:1000), p GSK3β (Cell Signaling 9336S 1:3000), GSK3β (BD Transduction 610201 1:3000), phosphoTau (Thr231, Invitrogen 44746G 1:5000), AT8 (Thermo #MN1020 1:2000), Tau-5 (Invitrogen AHB0042, 1:5000), pAkt (Ser473, Cell Signaling 9271S, 1:1000), Akt (Cell Signaling 9272S 1:1000), pERK (Cell Signaling 4370 1:1000), ERK (Cell Signaling 4696S 1:1000), pSrc (Biosource 44660 1:1000), Src (Biosource 44-656Z 1:1000), receptor-associated protein (RAP) (692 1:1000). Secondary antibodies used were: goat anti-mouse AlexaFluor 488 (Molecular Probes, 1:1000, for immunohistochemistry), donkey anti-rabbit ECL, HRP-linked (Amersham NA934 1:3000), and donkey anti-mouse 800 and donkey anti-rabbit 680 (Licor, 1:3000, for western blotting).

Lane-Donovan C., Philips G.T., Wasser C.R., Durakoglugil M.S., Masiulis I., Upadhaya A., Pohlkamp T., Coskun C., Kotti T., Steller L., Hammer R.E., Frotscher M., Bock H.H, & Herz J. (2015). Reelin protects against amyloid β toxicity in vivo. Science signaling, 8(384), ra67.

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Quantitative Analysis of Alzheimer's Biomarkers

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The information about chemicals and antibodies used in the method is as follows: charged aerosol detector (CAD) (Thermo Scientific, Waltham, MA, USA), 0.1% fomic acid (Sigma Aldrich, Saint Louis, MO, USA), acetonitrile (Sigma Aldrich), amyloid-β (Santa Cruz, Dallas, TX, USA, sc-53822, 1:500), BACE1 (Invitrogen, Waltham, MA, USA, PA5-19952, 10 μg/mL), APP (Invitrogen, 14-9749-82, 2.5 μg/mL), Iba-1 (Abcam, Cambridge, UK, ab178846, 1:2000), GFAP (BD Biosciences, Dickinson, ND, USA, BD-556328, 5 μg/mL), β-actin (Santa Cruz, sc-8432, 1:1000), phospho-Tau (Invitrogen, 44-752G, 1:1000), BACE1 (Invitrogen, PA5-19952, 1:1000), phospho-JNK (Santa Cruz, sc-6254, 1:1000), phospho-ERK (Santa Cruz, sc-7383, 1:1000), phospho-p38 (Cell Signaling, Danvers, MA, USA, csD3F9, 1:1000), anti-goat mouse (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SAB-100-J, 1:2000), and anti-goat rabbit (Enzo Life Sciences, ADI-SAB-300-J, 1:2000).

Lee W.S., Lee H.J., Yang J.Y., Shin H.L., Choi S.W., Kim J.K., Seo W.D, & Kim E.H. (2022). The Potential Neuroprotective Effects of Extracts from Oat Seedlings against Alzheimer’s Disease. Nutrients, 14(19), 4103.

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